NDLI: Electrochemical microfluidic biosensor for the detection of nucleic acid sequences

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Front cover

Contents

Inside front cover

Miniaturized continuous flow reaction vessels: influence on chemical reactions

Cell handling using microstructured membranes

A passive microfluidic hydrogen–air fuel cell with exceptional stability and high performance

An actuated pump on-chip powered by cultured cardiomyocytes

Phospholipid biotinylation of polydimethylsiloxane (PDMS) for protein immobilization

Free-flow zone electrophoresis and isoelectric focusing using a microfabricated glass device with ion permeable membranes

A three-channel microfluidic device for generating static linear gradients and its application to the quantitative analysis of bacterial chemotaxis

Characterization of a membrane-based gradient generator for use in cell-signaling studies

Microfluidic arrays for logarithmically perfused embryonic stem cell culture

Gelatin based microfluidic devices for cell culture

Electrochemical microfluidic biosensor for the detection of nucleic acid sequences

Microfluidic sorting system based on optical waveguide integration and diode laser bar trapping

Microfluidic approach for rapid multicomponent interfacial tensiometry

Formation of droplets and bubbles in a microfluidic T-junction—scaling and mechanism of break-up

Biomimetic design of microfluidic manifolds based on a generalised Murray's law

Back cover

Lab on a Chip Vol - 6 Issue - 2

Electrochemical microfluidic biosensor for the detection of nucleic acid sequences

Content Provider	Royal Society of Chemistry (RSC)
Author	Goral, Vasiliy N. Baeumner, Antje J. Zaytseva, Natalya V.
Copyright Year	2006
Abstract	A microfluidic biosensor with electrochemical detection for the quantification of nucleic acid sequences was developed. In contrast to most microbiosensors that are based on fluorescence for signal generation, it takes advantage of the simplicity and high sensitivity provided by an amperometric and coulorimetric detection system. An interdigitated ultramicroelectrode array (IDUA) was fabricated in a glass chip and integrated directly with microchannels made of poly(dimethylsiloxane) (PDMS). The assembly was packaged into a Plexiglas® housing providing fluid and electrical connections. IDUAs were characterized amperometrically and using cyclic voltammetry with respect to static and dynamic responses for the presence of a reversible redox couple—potassium hexacyanoferrate (II)/hexacyanoferrate (III) (ferri/ferrocyanide). A combined concentration of 0.5 µM of ferro/ferricyanide was determined as lower limit of detection with a dynamic range of 5 orders of magnitude. Background signals were negligible and the IDUA responded in a highly reversible manner to the injection of various volumes and various concentrations of the electrochemical marker. For the detection of nucleic acid sequences, liposomes entrapping the electrochemical marker were tagged with a DNA probe, and superparamagnetic beads were coated with a second DNA probe. A single stranded DNA target sequence hybridized with both probes. The sandwich was captured in the microfluidic channel just upstream of the IDUA via a magnet located in the outside housing. Liposomes were lysed using a detergent and the amount of released ferro/ferricyanide was quantified while passing by the IDUA. Optimal location of the magnet with respect to the IDUA was investigated, the effect of dextran sulfate on the hybridization reaction was studied and the amount of magnetic beads used in the assay was optimized. A dose response curve using varying concentrations of target DNA molecules was carried out demonstrating a limit of detection at 1 fmol assay−1 and a dynamic range between 1 and 50 fmol. The overall assay took 6 min to complete, plus 15–20 min of pre-incubation and required only a simple potentiostat for signal recording and interpretation.
Starting Page	414
Ending Page	421
Page Count	8
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	6
Issue Number	3
Journal	Lab on a Chip
DOI	10.1039/b513239h
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	IDUA Fluorescence Redox Dynamic range Poly(methyl methacrylate) Superparamagnetism Ultramicroelectrode Cyclic voltammetry Dextran Microchannel (microtechnology) Sandwich compound Protease Biosensor Hybridization probe DNA Potentiostat Electrochemistry Ferricyanide Sulfate Ferrocyanide Polydimethylsiloxane
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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