NDLI: 3D-templated, fully automated microfluidic input/output multiplexer for endocrine tissue culture and secretion sampling

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Lab on a Chip Vol -17

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Lab on a Chip Vol - 17 Issue - 2

Front cover

Inside front cover

Contents list

Controllable synthesis of functional nanoparticles by microfluidic platforms for biomedical applications – a review

An electrohydrodynamic technique for rapid mixing in stationary droplets on digital microfluidic platforms

Centrifugal micro-channel array droplet generation for highly parallel digital PCR

3D printed metal molds for hot embossing plastic microfluidic devices

Steering air bubbles with an add-on vacuum layer for biopolymer membrane biofabrication in PDMS microfluidics

Image processing and analysis system for development and use of free flow electrophoresis chips

Plasma free reversible and irreversible microfluidic bonding

In situ integration of graphene foam–titanium nitride based bio-scaffolds and microfluidic structures for soil nutrient sensors

Water-head-driven microfluidic oscillators for autonomous control of periodic flows and generation of aqueous two-phase system droplets

Xurography for microfluidics on a reactive solid

Slanted, asymmetric microfluidic lattices as size-selective sieves for continuous particle/cell sorting

Real-time observation of protein aggregates in pharmaceutical formulations using liquid cell electron microscopy

On-chip integration of droplet microfluidics and nanostructure-initiator mass spectrometry for enzyme screening

Development of a point-of-care diagnostic for influenza detection with antiviral treatment effectiveness indication

3D-templated, fully automated microfluidic input/output multiplexer for endocrine tissue culture and secretion sampling

Back cover

Lab on a Chip Vol - 17 Issue - 1

3D-templated, fully automated microfluidic input/output multiplexer for endocrine tissue culture and secretion sampling

Content Provider	Royal Society of Chemistry (RSC)
Author	Hu, Juan Li, Xiangpeng Brooks, Jessica C. Easley, Christopher J. Ford, Katarena I.
Copyright Year	2017
Abstract	A fully automated, 16-channel microfluidic input/output multiplexer (μMUX) has been developed for interfacing to primary cells and to improve understanding of the dynamics of endocrine tissue function. The device utilizes pressure driven push-up valves for precise manipulation of nutrient input and hormone output dynamics, allowing time resolved interrogation of the cells. The ability to alternate any of the 16 channels from input to output, and vice versa, provides for high experimental flexibility without the need to alter microchannel designs. 3D-printed interface templates were custom designed to sculpt the above-channel polydimethylsiloxane (PDMS) in microdevices, creating millimeter scale reservoirs and confinement chambers to interface primary murine islets and adipose tissue explants to the μMUX sampling channels. This μMUX device and control system was first programmed for dynamic studies of pancreatic islet function to collect ∼90 minute insulin secretion profiles from groups of ∼10 islets. The automated system was also operated in temporal stimulation and cell imaging mode. Adipose tissue explants were exposed to a temporal mimic of post-prandial insulin and glucose levels, while simultaneous switching between labeled and unlabeled free fatty acid permitted fluorescent imaging of fatty acid uptake dynamics in real time over a ∼2.5 hour period. Application with varying stimulation and sampling modes on multiple murine tissue types highlights the inherent flexibility of this novel, 3D-templated μMUX device. The tissue culture reservoirs and μMUX control components presented herein should be adaptable as individual modules in other microfluidic systems, such as organ-on-a-chip devices, and should be translatable to different tissues such as liver, heart, skeletal muscle, and others.
Starting Page	341
Ending Page	349
Page Count	9
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	17
Issue Number	2
Journal	Lab on a Chip
DOI	10.1039/c6lc01201a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Multiplexing Hormone Adipose tissue Endocrine system Liver Tissue culture Organ-on-a-chip Glucose Insulin Skeletal muscle Push-up Fatty acid Pancreatic islets Polydimethylsiloxane
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A Microfluidic Hanging-Drop-Based Islet Perifusion System for Studying Glucose-Stimulated Insulin Secretion From Multiple Individual Pancreatic Islets

Pivotal role of leptin in insulin effects.

Mechanistic Investigation of GHS-R Mediated Glucose-Stimulated Insulin Secretion in Pancreatic Islets

Estrogen increases glucose-induced insulin secretion from mouse pancreatic islets cultured in a prolonged high glucose.

Isolated mouse pancreatic islets in culture: Effects of serum and different culture media on the insulin production of the islets

WAT-on-a-chip: a physiologically relevant microfluidic system incorporating white adipose tissue

Tissue factor/factor VIIa signalling promotes cytokine-induced beta cell death and impairs glucose-stimulated insulin secretion from human pancreatic islets

Inhibition of cholinergic potentiation of insulin secretion from pancreatic islets by chronic elevation of glucose and fatty acids: Protection by casein kinase 2 inhibitor

Pancreatic Thyrotropin Releasing Hormone and Mechanism of Insulin Secretion

3D-templated, fully automated microfluidic input/output multiplexer for endocrine tissue culture and secretion sampling

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Pivotal role of leptin in insulin effects.

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Estrogen increases glucose-induced insulin secretion from mouse pancreatic islets cultured in a prolonged high glucose.

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3D-templated, fully automated microfluidic input/output multiplexer for endocrine tissue culture and secretion sampling