NDLI: Cell docking inside microwells within reversibly sealed microfluidic channels for fabricating multiphenotype cell arrays

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Comment on “AC frequency characteristics of coplanar impedance sensors as design parameters” by Jongin Hong, Dae Sung Yoon, Sung Kwan Kim, Tae Song Kim, Sanghyo Kim, Eugene Y. Pak and Kwangsoo No, Lab Chip, 2005, 5, 270

Reply to ‘Comment on “AC frequency characteristics of coplanar impedance sensors as design parameters”’ by Pontus Linderholm and Philippe Renaud, Lab Chip, 2005, 5, DOI: 10.1039/b512077b

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Cell docking inside microwells within reversibly sealed microfluidic channels for fabricating multiphenotype cell arrays

Content Provider	Royal Society of Chemistry (RSC)
Author	Eng, George Yeh, Judy Farokhzad, Omid C. Kaji, Hirokazu Borenstein, Jeffrey Karp, Jeffrey Langer, Robert Khademhosseini, Ali
Copyright Year	2005
Abstract	We present a soft lithographic method to fabricate multiphenotype cell arrays by capturing cells within an array of reversibly sealed microfluidic channels. The technique uses reversible sealing of elastomeric polydimethylsiloxane (PDMS) molds on surfaces to sequentially deliver various fluids or cells onto specific locations on a substrate. Microwells on the substrate were used to capture and immobilize cells within low shear stress regions inside channels. By using an array of channels it was possible to deposit multiple cell types, such as hepatocytes, fibroblasts, and embryonic stem cells, on the substrates. Upon formation of the cell arrays on the substrate, the PDMS mold could be removed, generating a multiphenotype array of cells. In addition, the orthogonal alignment and subsequent attachment of a secondary array of channels on the patterned substrates could be used to deliver fluids to the patterned cells. The ability to position many cell types on particular regions within a two dimensional substrate could potentially lead to improved high-throughput methods applicable to drug screening and tissue engineering.
Starting Page	1380
Ending Page	1386
Page Count	7
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	5
Issue Number	12
Journal	Lab on a Chip
DOI	10.1039/b508096g
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	Hepatocyte Tissue engineering Shear stress The Technique Orthogonality Polydimethylsiloxane
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

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Microcirculation within grooved substrates regulates cell positioning and cell docking inside microfluidic channels

Microcirculation within Grooved Substrates regulates Cell Positioning and Cell Docking inside Microfluidic Channels

A computational and experimental study inside microfluidic systems: the role of shear stress and flow recirculation in cell docking

Hydrodynamic simulation of cell docking in microfluidic channels with different dam structures

Biological implications of polydimethylsiloxane-based microfluidic cell culture†

Glass coating for PDMS microfluidic channels by sol–gel methods

Molded polyethylene glycol microstructures for capturing cells within microfluidic channels

Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays

A computational and experimental study inside microfluidic systems: the role of shear stress and flow recirculation in cell docking

Cell docking inside microwells within reversibly sealed microfluidic channels for fabricating multiphenotype cell arrays

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