NDLI: Microfluorimeter with disposable polymer chip for detection of coeliac disease toxic gliadin

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Lab on a Chip Vol -17

Lab on a Chip Vol -9

Lab on a Chip Vol - 9 Issue - 24

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Inside front cover

Contents and Highlights in Chemical Technology

Research Highlights ( Lab on a Chip Vol - 9 Issue - 24 )

PCR amplification and genetic analysis in a microwell cell culturing chip

Field-effect based attomole titrations in nanoconfinement

Room-temperature intermediate layer bonding for microfluidic devices

“Print-n-Shrink” technology for the rapid production of microfluidic chips and protein microarrays

A blood sampling microsystem for pharmacokinetic applications: Design, fabrication, and initial results

Rapid integrated biosensor for multiplexed immunoassays based on actuated magnetic nanoparticles

High-pressure on-chip mechanical valves for thermoplastic microfluidic devices

Correlating short-term Ca²⁺ responses with long-term protein expression after activation of single T cells

2D-PCR: a method of mapping DNA in tissue sections

Microfluorimeter with disposable polymer chip for detection of coeliac disease toxic gliadin

Rapid protein depletion from complex samples using a bead-based microfluidic device for the point of care

Detection of viruses with molecularly imprinted polymers integrated on a microfluidic biochip using contact-less dielectric microsensors

Highly parallel introduction of nucleic acids into mammalian cells grown in microwell arrays

Sequential processing from cell lysis to protein assay on a chip enabling the optimization of an F₁-ATPase single molecule assay condition

Multifunctional plasmonic sensors on low-cost subwavelength metallic nanoholes arrays

Rapid multicomponent optical protein patterning

Micro contactor based on isotachophoretic sample transport

An automated, pre-programmed, multiplexed, hydraulic microvalve

Centrifugo-pneumatic valve for metering of highly wetting liquids on centrifugal microfluidic platforms

Back matter

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Lab on a Chip Vol - 9 Issue - 19

Lab on a Chip Vol - 9 Issue - 9

Lab on a Chip Vol - 9 Issue - 8

Lab on a Chip Vol - 9 Issue - 7

Lab on a Chip Vol - 9 Issue - 6

Lab on a Chip Vol - 9 Issue - 5

Lab on a Chip Vol - 9 Issue - 4

Lab on a Chip Vol - 9 Issue - 3

Microfluorimeter with disposable polymer chip for detection of coeliac disease toxic gliadin

Content Provider	Royal Society of Chemistry (RSC)
Author	Frese, Ines Sullivan, Ciara K. O' Mairal, Teresa Drese, Klaus Katakis, Ioanis Llaudet, Enrique Redondo, Carmen Bermudo Germar, Frithjof
Copyright Year	2009
Abstract	Coeliac disease is an inflammatory disease of the upper small intestine and results from gluten ingestion in genetically susceptible individuals, and is the only life-long nutrient-induced enteropathy. The only treatment is a strict gluten-free diet and the longer the individual fails to adhere to this diet, the greater the chance of developing malnutrition and other complications. The existence of reliable gluten free food is crucial to the well-being of the population. Here we report on a microfluorimeter device for the in situdetection of gliadin in foodstuffs, which could be used for a rapid control of raw materials in food processing, as well as for process control of gliadin contamination. The microfluorimeter is based on a reflector that is used inside a microfluidic chip, exploiting various strategically placed reflective or totally metallised mirrors for efficient collection of the fluorescent light emitted in a large solid angle. The chip is capable of executing five assays in parallel and has been demonstrated to possess detection sensitivity applicable to fluoroimmunoassays. Various immunoassay formats exploiting fluorescence detection, using enzyme/fluorophore labels were developed and compared in terms of sensitivity, ease of assay, assay time and compatibility with buffer used to extract gliadin from raw and cooked foodstuffs, with the best performance observed with an indirect competition assay using a fluorophore-labelled anti-mouse antibody. This assay was exploited within the microfluorimeter device, and a very low detection limit of 4.1 ng/mL was obtained. The system was observed to be highly reproducible, with an RSD of 5.9%, for a concentration of 50 ng/mL of gliadin applied to each of the five channels of the microfluorimeter. Biofunctionalised disposable strips incorporated into the microfluorimeter were subjected to accelerated Arrhenius thermal stability studies and it was demonstrated that strips pre-coated with gliadin could be stored for ≈ 2 years at 4 °C, with no discernable loss in sensitivity or detectability of the assay. Finally, the microfluorimeter was applied to the analysis of commercial gluten-free food samples, and an excellent correlation with routine ELISA measurements was obtained. The developed microfluorimeter should find widespread application for on-site execution of fluoroimmunoassays.
Starting Page	3535
Ending Page	3542
Page Count	8
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	9
Issue Number	24
Journal	Lab on a Chip
DOI	10.1039/b914635k
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Immunoassay Enzyme Process control Fluorescence Enteropathy Coeliac disease Small intestine Arrhenius Solid angle Fluorophore Gliadin Gluten-free diet Arrhenius equation Malnutrition Gluten
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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Microfluorimeter with disposable polymer chip for detection of coeliac disease toxic gliadin

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Persistence of elevated deamidated gliadin peptide antibodies on a gluten-free diet indicates nonresponsive coeliac disease.

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Microfluorimeter with disposable polymer chip for detection of coeliac disease toxic gliadin