NDLI: Photopolymerized diffusion-defined polyacrylamide gradient gels for on-chip protein sizing

Please wait, while we are loading the content...

Engineering of PDMS surfaces for use in microsystems for capture and isolation of complex and biomedically important proteins: Epidermal growth factor receptor as a model system

Microfluidic assembly blocks

SmartBuild–A truly plug-n-play modular microfluidic system

Soft lithography: masters on demand

Enrichment of putative stem cells from adipose tissue using dielectrophoretic field-flow fractionation

Ultraviolet-based bonding for perfluoropolyether low aspect-ratio microchannels and hybrid devices

Concentration control for protein crystallization via a continuously-fed crystallization chamber

Maskless photolithography using UV LEDs

Back matter

Back cover

Lab on a Chip Vol - 8 Issue - 7

Lab on a Chip Vol - 8 Issue - 6

Lab on a Chip Vol - 8 Issue - 5

Lab on a Chip Vol - 8 Issue - 4

Lab on a Chip Vol - 8 Issue - 3

Lab on a Chip Vol - 8 Issue - 2

Photopolymerized diffusion-defined polyacrylamide gradient gels for on-chip protein sizing

Content Provider	Royal Society of Chemistry (RSC)
Author	Lo, Catherine T. Throckmorton, Daniel J. Herr, Amy E. Singh, Anup K.
Copyright Year	2008
Abstract	We report on a facile diffusion-based photopatterning technique for generating linear and non-linear decreasing pore-size gradients in cross-linked polyacrylamide gels. Diffusion of low viscosity polymer precursor solutions and a two-step photopatterning process were used to define the decreasing pore-size gradient gels in a microfluidic format, thus eliminating the need for controlled mixing and delivery of polymer precursor solutions. We present an analytical model of the non-steady state diffusion process and numerically evaluate that model for direct comparison with empirical characterizations of the gradient gels. We show that the analytical model provides an effective means to predict the steepness and linearity of a desired gradient gel prior to fabrication. To assess electrophoretic assay performance in the microfluidic gradient gels, on-chip sizing of protein samples (20–116 kDa) was investigated. Baseline resolution of six proteins was demonstrated in 4 s using 3.5% to 10% polyacrylamide gradient gels. The demonstrated ability to conduct efficient protein sizing in ultra-short separation lengths (0.3 cm) means low applied electric potentials are needed to achieve the electric field strengths required for protein separations. The low required electric potentials relax operating constraints on electrical components, as is especially important for translation of the assay into pre-clinical and clinical settings. The gradient gel fabrication method reported is amenable to adaptation to non-sizing protein assays, as well as integration with upstream sample preparation steps and subsequent orthogonal downstream assays.
Starting Page	1273
Ending Page	1279
Page Count	7
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	8
Issue Number	8
Journal	Lab on a Chip
DOI	10.1039/b804485f
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Viscosity Electric field Polyacrylamide Polymer Electrophoresis Diffusion Orthogonality Protein
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Photopolymerized cross-linked polyacrylamide gels for on-chip protein sizing.

Microchannel protein separation by electric field gradient focusing

Integrated polymer chip for two-dimensional capillary gel electrophoresis

Integration of isoelectric focusing with multi-channel gel electrophoresis by using microfluidic pseudo-valves

Microfluidic 2-D PAGE using multifunctional in situ polyacrylamide gels and discontinuous buffers

Electrophoresis in microfabricated devices using photopolymerized polyacrylamide gels and electrode-defined sample injection

Rapid and variable-volume sample loading in sieving electrophoresis microchips using negative pressure combined with electrokinetic force

Electrostatic induction of the electric field into free-flow electrophoresis devices

Continuous dielectrophoretic cell separation microfluidic device

Photopolymerized diffusion-defined polyacrylamide gradient gels for on-chip protein sizing

Similar Documents

Photopolymerized cross-linked polyacrylamide gels for on-chip protein sizing.

Microchannel protein separation by electric field gradient focusing

Integrated polymer chip for two-dimensional capillary gel electrophoresis

Integration of isoelectric focusing with multi-channel gel electrophoresis by using microfluidic pseudo-valves

Microfluidic 2-D PAGE using multifunctional in situ polyacrylamide gels and discontinuous buffers

Electrophoresis in microfabricated devices using photopolymerized polyacrylamide gels and electrode-defined sample injection

Rapid and variable-volume sample loading in sieving electrophoresis microchips using negative pressure combined with electrokinetic force

Electrostatic induction of the electric field into free-flow electrophoresis devices

Continuous dielectrophoretic cell separation microfluidic device

Photopolymerized diffusion-defined polyacrylamide gradient gels for on-chip protein sizing