NDLI: Gene transfer device utilizing micron-spiked electrodes produced by the self-organization phenomenon of Fe-alloy

Content Provider	Royal Society of Chemistry (RSC)
Author	Tsumori, Fujio Kotera, Hidetoshi Miyano, Naoki Inoue, Yuuki Fujii, Keisuke Iwata, Hiroo Teramura, Yuji
Copyright Year	2008
Abstract	In the diffusional phase transformation of two-phase alloys, the new phase precipitates form the matrix phase at specific temperatures, followed by the formation of a mixed microstructure comprising the precipitate and the matrix. It has been found that by specific chemical-etching treatment, the precipitate in Fe-25Cr-6Ni alloy projects substantially and clusters at the surface. The configuration of the precipitate has an extremely high aspect ratio: it is several microns in width and several tens of microns in length (known as micron-spiked). This study targets the development of a gene transfer device with a micro-spike produced based on the self-organization phenomenon of the Fe-25Cr-6Ni alloy. With this spike-projected device, we tried to efficiently transfer plasmid DNA into adherent cells by electric pulse-triggered gene transfer using a plasmid-loaded electrode (electroporation-based reverse transfection). The spiked structure was applied to a substrate of the device to allow efficient gene transfer into adherent cells, although the general substrate was flat and had a smooth surface. The results suggest that this unique spike-projected device has potential applications in gene transfer devices for the analysis of the human genome in the post-genome period.
Starting Page	1104
Ending Page	1109
Page Count	6
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	8
Issue Number	7
Journal	Lab on a Chip
DOI	10.1039/b718655j
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Plasmid Electrode Gene Human genome DNA Composite material Reverse transfection Self-organization
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

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