NDLI: Model-controlled hydrodynamic focusing to generate multiple overlapping gradients of surface-immobilized proteins in microfluidic devices

Content Provider	Royal Society of Chemistry (RSC)
Author	Wikswo, John P. Anderson, Alexander R. A. Quaranta, Vito Estrada, Lourdes Georgescu, Walter Jourquin, Jerome
Copyright Year	2008
Abstract	Historically, it has been difficult to generate accurate and reproducible protein gradients for studies of interactions between cells and extracellular matrix. Here we demonstrate a method for rapid patterning of protein gradients using computer-driven hydrodynamic focusing in a simple microfluidic device. In contrast to published work, we are moving the complexity of gradient creation from the microfluidic hardware to dynamic computer control. Using our method, switching from one gradient profile to another requires only a few hours to devise a new control file, not days or weeks to design and build a new microfluidic device. Fitting existing protein deposition models to our data, we can extract key parameters needed for controlling protein deposition. Several protein deposition models were evaluated under microfluidic flow conditions. A mathematical model for our deposition method allows us to determine the parameters for a protein adsorption model and then predict the final shape of the surface density gradient. Simple and non-monotonic single and multi-protein gradient profiles were designed and deposited using the same device.
Starting Page	238
Ending Page	244
Page Count	7
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	8
Issue Number	2
Journal	Lab on a Chip
DOI	10.1039/b716203k
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Adsorption Extracellular matrix Mathematical model Protein
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

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