NDLI: Stability, cytotoxicity and cell uptake of water-soluble dendron–conjugated gold nanoparticles with 3, 12 and 17 nm cores

Content Provider	Royal Society of Chemistry (RSC)
Author	Deol, Suprit Weerasuriya, Nisala Shon, Young-Seok
Copyright Year	2015
Abstract	This article describes the synthesis of water-soluble dendron–conjugated gold nanoparticles (Den–AuNPs) with various average core sizes and the evaluation of stability, cytotoxicity, cell permeability and uptake of these materials. The characterization of Den–AuNPs using various techniques including transmission electron microscopy (TEM), matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS), 1H NMR, FT-IR, and UV-vis spectroscopy confirms the dendron conjugation to the glutathione-capped gold nanoparticles (AuNPs). The stability of AuNPs and Den–AuNPs in solutions of different pH and salt concentration is determined by monitoring the changes in surface plasmon bands of gold using UV-vis spectroscopy. The stability of Den–AuNPs at different pH remained about the same compared to that of AuNPs. In comparison, the Den–AuNPs are found to be more stable than the precursor AuNPs maintaining their solubility in the aqueous solution with the salt concentration of up to 100 mM. The improved stability of Den–AuNPs suggests that the post-functionalization of thiol-capped gold nanoparticle surfaces with dendrons can further improve the physiological stability and biocompatibility of gold nanoparticle-based materials. Cytotoxicity studies of AuNPs and Den–AuNPs with and without fluorophores are also performed by examining cell viability for 3T3 fibroblasts using a MTT cell proliferation assay. The conjugation of dendrons to the AuNPs with a fluorophore is able to decrease the cytotoxicity brought about by the fluorophore. The successful uptake of Den–AuNPs in mouse fibroblast 3T3 cells shows the physiological viability of the hybrid materials.
Starting Page	6071
Ending Page	6080
Page Count	10
File Format	HTM / HTML PDF
ISSN	2050750X
Volume Number	3
Issue Number	29
Journal	Journal of Materials Chemistry B
DOI	10.1039/c5tb00608b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	Cytotoxicity Semipermeable membrane Transmission electron microscopy Laser Mass spectrometry Nuclear magnetic resonance Fourier-transform infrared spectroscopy Ultraviolet\u2013visible spectroscopy PH Surface plasmon Nanoparticle Biocompatibility Fluorophore Laboratory mouse Fibroblast
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Materials Science Biomedical Engineering

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