NDLI: DNA protection against ultraviolet irradiation by encapsulation in a multilayered SiO<sub>2</sub>/TiO<sub>2</sub> assembly

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DNA protection against ultraviolet irradiation by encapsulation in a multilayered SiO₂/TiO₂ assembly

Content Provider	Royal Society of Chemistry (RSC)
Author	Puddu, M. Grass, R. N. Paunescu, D. Mora, C. A. Krumeich, F.
Copyright Year	2014
Abstract	DNA is protected against UV-induced damage by encapsulation in a core–shell–shell particulate construct. The DNA is hermetically sealed in SiO2 particles coated with TiO2. The TiO2 coating acts as a physical sunscreen and prevents high energy photons from damaging the nucleic acids. DNA can be recovered unharmed from the protection system with fluoride comprising buffers, and then directly analyzed using biochemical standard techniques (quantitative PCR, gel electrophoresis and Sanger sequencing). The coatings increase the DNA UV resistance by 42 times, which is equivalent to the increase in UV resistance obtained by bacteria during sporulation. The attenuation coefficient of the 20 nm titania layer is 1.8 106 cm−1 at 254 nm UV irradiation and optical attenuation is largely attributed to light scattering on the titania surface.
Starting Page	8504
Ending Page	8509
Page Count	6
File Format	HTM / HTML PDF
ISSN	2050750X
Volume Number	2
Issue Number	48
Journal	Journal of Materials Chemistry B
DOI	10.1039/c4tb01552e
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Sanger DNA Sunscreen Fluoride Real-time polymerase chain reaction Gel electrophoresis Sanger sequencing Bacteria Attenuation coefficient Scattering
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Materials Science Biomedical Engineering

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