NDLI: Lipophilic and cationic triphenylphosphonium grafted linear polyethylenimine polymers for efficient gene delivery to mammalian cells

Content Provider	Royal Society of Chemistry (RSC)
Author	Gupta, K. C. Bansal, R. Kumar, P. Tripathi, S. K.
Copyright Year	2012
Abstract	Synthetic chemical vectors have recently provided a versatile and robust platform for the safe and efficient delivery of exogenous genes. Here, for the first time, a small series of N-butyltriphenylphosphonium bromide-grafted-linear polyethylenimine (BTP-g-lP) polymers (N–P hybrid polymers) have been evaluated for their ability to deliver genes into mammalian cell lines, viz., MCF-7 and A549 cells. Biophysical characterization revealed that the projected polymers efficiently interacted with plasmid DNA, and the resulting complexes displayed hydrodynamic diameters in the range of 249–307 nm with relatively higher zeta potential values of +31 to +34 mV (cf. lPEI, +26 mV). The tethering of lipophilic and cationic triphenylphosphonium moieties to linear PEI (lPEI) addressed several limitations associated with lPEI, such as solubility, the stability of the pDNA complexes and the timely release of pDNA for nuclear localization as assessed by protection and release assays. Also, the lipophilic interactions between cellular membranes and the pDNA complexes mediated the efficient cellular uptake and internalization of the pDNA complexes, resulting in significantly higher transfection efficiency in these cell lines, outperforming the GenePORTER 2™, Lipofectamine™ and Superfect™ used in the study for comparison purposes. Confocal studies using dual-labeled TMR-BTP-g-lP3/YOYO-1-pDNA complex in MCF-7 cells confirmed that the complex behaved more or less like native lPEI, as the substitution of the phosphonium moiety was too small to affect the intracellular trafficking. Furthermore, the versatility of the BTP-g-lP3 vector was established by GFP specific siRNA delivery, which resulted in ∼79% suppression of targeted gene expression (cf. Lipofectamine™, ∼55%). Altogether, the study demonstrates the potential of these hybrid polymers for the efficient delivery of nucleic acids for future gene therapy applications.
Starting Page	25427
Ending Page	25436
Page Count	10
File Format	HTM / HTML PDF
ISSN	09599428
Volume Number	22
Issue Number	48
Journal	Journal of Materials Chemistry
DOI	10.1039/c2jm35243e
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Biophysical GFP Polyethylenimine MCF-7 Plasmid DNA Zeta potential Lipophilicity Transfection Phosphonium Euclidean vector Green fluorescent protein Small interfering RNA Gene Gene therapy
Content Type	Text
Resource Type	Article
Subject	Chemistry Materials Chemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Lipophilic and cationic triphenylphosphonium grafted linear polyethylenimine polymers for efficient gene delivery to mammalian cells

Amphiphilic polyethylenimine polymers mediate efficient delivery of DNA and siRNA in mammalian cells

Hydrophobic and membrane permeable polyethylenimine nanoparticles efficiently deliver nucleic acids in vitro and in vivo

Efficient gene delivery into cells by a surprisingly small three-armed peptide ligand

Synthesis and evaluation of N-(2,3-dihydroxypropyl)-PEIs as efficient vectors for nucleic acids

Exploring low-positively charged thermosensitive copolymers as gene delivery vectors

Polyethyleneimine-grafted poly(N-3-hydroxypropyl)aspartamide as a biodegradable gene vector for efficient gene transfection

Mixing-sequence-dependent nucleic acid complexation and gene transfer efficiency by polyethylenimine

Cationic lipo-thiophosphoramidates for gene delivery: synthesis, physico-chemical characterization and gene transfection activity – comparison with lipo-phosphoramidates

Lipophilic and cationic triphenylphosphonium grafted linear polyethylenimine polymers for efficient gene delivery to mammalian cells

Similar Documents

Lipophilic and cationic triphenylphosphonium grafted linear polyethylenimine polymers for efficient gene delivery to mammalian cells

Amphiphilic polyethylenimine polymers mediate efficient delivery of DNA and siRNA in mammalian cells

Hydrophobic and membrane permeable polyethylenimine nanoparticles efficiently deliver nucleic acids in vitro and in vivo

Efficient gene delivery into cells by a surprisingly small three-armed peptide ligand

Synthesis and evaluation of N-(2,3-dihydroxypropyl)-PEIs as efficient vectors for nucleic acids

Exploring low-positively charged thermosensitive copolymers as gene delivery vectors

Polyethyleneimine-grafted poly(N-3-hydroxypropyl)aspartamide as a biodegradable gene vector for efficient gene transfection

Mixing-sequence-dependent nucleic acid complexation and gene transfer efficiency by polyethylenimine

Cationic lipo-thiophosphoramidates for gene delivery: synthesis, physico-chemical characterization and gene transfection activity – comparison with lipo-phosphoramidates

Lipophilic and cationic triphenylphosphonium grafted linear polyethylenimine polymers for efficient gene delivery to mammalian cells