NDLI: Speciation analysis of sugar phosphates <i>via</i> anion exchange chromatography combined with inductively coupled plasma dynamic reaction cell mass spectrometry – optimization for the analysis of yeast cell extracts

Please wait, while we are loading the content...

On-line flow injection solid phase extraction using oxidised carbon nanotubes as the substrate for cold vapour-atomic absorption determination of Hg(ii) in different kinds of water

Enhancement of intensity in microwave-assisted laser-induced breakdown spectroscopy for remote analysis of nuclear fuel recycling

Coupling of cold vapor generation with an atmospheric pressure glow microdischarge sustained between a miniature flow helium jet and a flowing liquid cathode for the determination of mercury by optical emission spectrometry

Matrix effects during laser ablation MC ICP-MS analysis of boron isotopes in tourmaline

Speciation analysis of sugar phosphates via anion exchange chromatography combined with inductively coupled plasma dynamic reaction cell mass spectrometry – optimization for the analysis of yeast cell extracts

Determination of lead isotope ratios in environmental matrices by quadrupole ICP-MS working at low sample consumption rates

Differencing as a method to estimate the uncertainty of a transient LA-ICPMS signal

Back cover

Journal of Analytical Atomic Spectrometry Vol - 29 Issue - 4

Journal of Analytical Atomic Spectrometry Vol - 29 Issue - 3

Journal of Analytical Atomic Spectrometry Vol - 29 Issue - 2

Journal of Analytical Atomic Spectrometry Vol -19

Journal of Analytical Atomic Spectrometry Vol -11

Journal of Analytical Atomic Spectrometry Vol -9

Journal of Analytical Atomic Spectrometry Vol -8

Journal of Analytical Atomic Spectrometry Vol -7

Journal of Analytical Atomic Spectrometry Vol -6

Journal of Analytical Atomic Spectrometry Vol -5

Journal of Analytical Atomic Spectrometry Vol -4

Journal of Analytical Atomic Spectrometry Vol -2

Speciation analysis of sugar phosphates via anion exchange chromatography combined with inductively coupled plasma dynamic reaction cell mass spectrometry – optimization for the analysis of yeast cell extracts

Content Provider	Royal Society of Chemistry (RSC)
Author	Klavins, Kristaps Koellensperger, Gunda Chu, Dinh Binh Hann, Stephan
Copyright Year	2014
Abstract	Anion exchange chromatography combined with inductively coupled plasma mass spectrometry was introduced and optimized for the separation and accurate quantification of sugar phosphates in cell extracts. Sugar phosphates have been separated on an anion exchange quaternary ammonium functionalized stationary phase (Dionex CarboPAC PA1) via gradient elution with 160 mM Na2CO3 and 50 mM NaOH. After separation, the sugar phosphates were on-line transferred to an inductively coupled plasma mass spectrometer after having passed an anion self-regenerating suppressor. Detection was performed via phosphorus as PO+ (m/z 47) was generated by the dynamic reaction cell technique using oxygen as a reaction gas. The ionization suppression process in the inductively coupled plasma caused by the high buffer strength of the chromatographic gradient was assessed by post-column flow injection analysis. It could be shown that the anion self-regenerating suppressor led to a significant reduction of signal suppression. With the new methodology excellent relative standard deviations of retention times of sugar phosphates for short- and long-term measurements were achieved. The relative standard deviations of short-term and long-term repeatability of peak areas were below 3.0 and 10.0% respectively. The absolute on-column LODs and LOQs of the optimized method were in the sub-picomole range. The developed method was applied for the purity assessment of commercially available sugar phosphate standards and evaluated for the quantification of sugar phosphates in yeast samples (Pichia pastoris) after extraction with boiling ethanol. Due to the lack of reference materials, the method accuracy was validated by method inter-comparison with an LCxLC-ESI-MS/MS based method, which has been developed in our laboratory.
Starting Page	915
Ending Page	925
Page Count	11
File Format	HTM / HTML PDF
ISSN	02679477
Volume Number	29
Issue Number	5
Journal	Journal of Analytical Atomic Spectrometry
DOI	10.1039/c4ja00043a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Dionex Chromatography Inductively coupled plasma mass spectrometry Sugar phosphates Ion Ammonium Inductively coupled plasma Mass spectrometry Phosphorus Oxygen Ionization Flow injection analysis Sucrose Phosphate Yeast Pichia pastoris Ethanol
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Analytical Chemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Development of anion-exchange high-performance liquid chromatography-inductively coupled plasma mass spectrometry methods for the speciation analysis of inorganic selenium and iodine in groundwater

Sample preparation strategies for quantitative analysis of catalase in red blood cells by elemental mass spectrometry

Quantitative determination of DNA adducts using liquid chromatography/electrospray ionization mass spectrometry and liquid chromatography/high-resolution inductively coupled plasma mass spectrometry

Evaluation of low pressure inductively coupled plasma mass spectrometry for the analysis of gaseous samples

Chlorine speciation analysis in blood by ion chromatography-inductively coupled plasma mass spectrometry

Speciation of metal–EDTA complexes by flow injection analysis with electrospray ionization mass spectrometry and ion chromatography with inductively coupled plasma mass spectrometry

Simultaneous speciation and determination of arsenic, chromium and cadmium in water samples by high performance liquid chromatography with inductively coupled plasma mass spectrometry

Sobeck & ebeling: mass spectrometric analysis for phosphate in soil extracts 1 mass spectrometric analysis for phosphate in soil extracts; comparison of mass spectrometry, colorimetry, and inductively coupled plasma.

Univariate optimization of segmented-flow injection for inductively coupled plasma mass spectrometry

Speciation analysis of sugar phosphates via anion exchange chromatography combined with inductively coupled plasma dynamic reaction cell mass spectrometry – optimization for the analysis of yeast cell extracts

Similar Documents

Development of anion-exchange high-performance liquid chromatography-inductively coupled plasma mass spectrometry methods for the speciation analysis of inorganic selenium and iodine in groundwater

Sample preparation strategies for quantitative analysis of catalase in red blood cells by elemental mass spectrometry

Quantitative determination of DNA adducts using liquid chromatography/electrospray ionization mass spectrometry and liquid chromatography/high-resolution inductively coupled plasma mass spectrometry

Evaluation of low pressure inductively coupled plasma mass spectrometry for the analysis of gaseous samples

Chlorine speciation analysis in blood by ion chromatography-inductively coupled plasma mass spectrometry

Speciation of metal–EDTA complexes by flow injection analysis with electrospray ionization mass spectrometry and ion chromatography with inductively coupled plasma mass spectrometry

Simultaneous speciation and determination of arsenic, chromium and cadmium in water samples by high performance liquid chromatography with inductively coupled plasma mass spectrometry

Sobeck & ebeling: mass spectrometric analysis for phosphate in soil extracts 1 mass spectrometric analysis for phosphate in soil extracts; comparison of mass spectrometry, colorimetry, and inductively coupled plasma.

Univariate optimization of segmented-flow injection for inductively coupled plasma mass spectrometry

Speciation analysis of sugar phosphates via anion exchange chromatography combined with inductively coupled plasma dynamic reaction cell mass spectrometry – optimization for the analysis of yeast cell extracts