NDLI: High-precision isotopic analysis of Cu in blood serum <i>via</i> multi-collector ICP-mass spectrometry for clinical investigation: steps towards improved robustness and higher sample throughput

Please wait, while we are loading the content...

Superior spatial resolution in confocal X-ray techniques using collimating channel array optics: elemental mapping and speciation in archaeological human bone

Evaluation of the use of cold plasma conditions for Fe isotopic analysis via multi-collector ICP-mass spectrometry: effect on spectral interferences and instrumental mass discrimination

Characterization of a new ICP-TOFMS instrument with continuous and discrete introduction of solutions

GEOTRACES inter-calibration of the stable silicon isotope composition of dissolved silicic acid in seawater

ID-TIMS U–Pb geochronology at the 0.1‰ level using 10¹³Ω resistors and simultaneous U and ¹⁸O/¹⁶O isotope ratio determination for accurate UO₂ interference correction

A dried droplet calibration approach for the analysis of solid samples through laser ablation – inductively coupled plasma mass spectrometry

High-precision isotopic analysis of Cu in blood serum via multi-collector ICP-mass spectrometry for clinical investigation: steps towards improved robustness and higher sample throughput

Detection of trace substances adhered to a metal surface by laser-induced breakdown spectroscopy

Size and metal composition characterization of nano- and microparticles in tattoo inks by a combination of analytical techniques

Accuracy improvement of quantitative LIBS analysis using wavelet threshold de-noising

Matrix dependency for oxide production rates by LA-ICP-MS

A new method for high-precision palladium isotope analyses of iron meteorites and other metal samples

Spectral emission enhancement by an electric pulse for LIBS and LAMIS

Quantitative bioimaging of Ca, Fe, Cu and Zn in breast cancer tissues by LA-ICP-MS

The online coupling of high performance liquid chromatography with atomic fluorescence spectrometry based on dielectric barrier discharge induced chemical vapor generation for the speciation of mercury

In situ²⁰⁷Pb/²⁰⁶Pb isotope ratio measurements using two Daly detectors equipped on an ICP-mass spectrometer

Back cover

Journal of Analytical Atomic Spectrometry Vol - 32 Issue - 2

Journal of Analytical Atomic Spectrometry Vol - 32 Issue - 1

Journal of Analytical Atomic Spectrometry Vol -31

Journal of Analytical Atomic Spectrometry Vol -29

Journal of Analytical Atomic Spectrometry Vol -19

Journal of Analytical Atomic Spectrometry Vol -11

Journal of Analytical Atomic Spectrometry Vol -9

Journal of Analytical Atomic Spectrometry Vol -8

Journal of Analytical Atomic Spectrometry Vol -7

Journal of Analytical Atomic Spectrometry Vol -6

Journal of Analytical Atomic Spectrometry Vol -5

Journal of Analytical Atomic Spectrometry Vol -4

Journal of Analytical Atomic Spectrometry Vol -2

High-precision isotopic analysis of Cu in blood serum via multi-collector ICP-mass spectrometry for clinical investigation: steps towards improved robustness and higher sample throughput

Content Provider	Royal Society of Chemistry (RSC)
Author	Vanhaecke, Frank Vlierberghe, Hans Van Lauwens, Sara Costas-Rodríguez, Marta
Copyright Year	2017
Abstract	High-precision isotopic analysis of Cu in blood serum via multi-collector ICP-mass spectrometry (MC-ICP-MS) has recently been shown valuable for diagnosis and prognosis of diseases affecting Cu metabolism. However, as a result of the low Cu concentration (≤2 mg L−1) in blood serum compared to the high contents of concomitant matrix elements (e.g., ∼3000 mg L−1 Na, ∼1000 mg L−1 S, ∼100 mg L−1 Ca and ∼20 mg L−1 Mg) and the measurable effect that the matrix typically exerts on the extent of instrumental mass discrimination in MC-ICP-MS, an exhaustive sample preparation protocol is required. In this work, an alternative procedure for Cu isolation from serum prior to isotope ratio measurement by MC-ICP-MS was evaluated. This procedure involves removal of the alkali and earth alkaline metals using 1 mL of Chelex-100 chelating ion exchange resin, followed by further purification of the Cu-fraction using 250 μL of AG MP1 anion exchange resin. The matrix elements were removed efficiently and Cu recoveries were quantitative in all cases. The alternative Cu isolation procedure showed several advantages compared to the conventional procedure, e.g., (i) a reduction in the amount of acids, cost and time required and (ii) lower blank levels. The effect of (remaining) concomitant matrix elements on the Cu isotope ratio accuracy and precision attainable was evaluated using both the standard interface (combination of standard cones and a large dry interface pump) and the high-transmission ‘jet’ interface (combination of ‘jet’ cones and a large dry interface pump). For standard solutions, the jet interface provided a superior internal precision (improved by a factor of 1.5) for the raw (uncorrected) 65Cu/63Cu ratio and a mitigated extent of instrumental mass discrimination. However, the influence of matrix elements on the Cu isotope ratio results was stronger with the jet interface than with the standard interface. The proposed procedure was validated using standards with known isotopic composition, a serum reference material and human serum samples. No statistical differences were obtained (at a 95% confidence interval) in the δ65Cu values using the proposed and the conventional (two column passes using 1 mL of AG MP1 resin) procedure. Finally, serum samples collected at different time points during a day from five volunteers were analysed in order to study diurnal variations in the serum Cu isotopic composition. No diurnal variations were established.
Starting Page	597
Ending Page	608
Page Count	12
File Format	HTM / HTML PDF
ISSN	02679477
Volume Number	32
Issue Number	3
Journal	Journal of Analytical Atomic Spectrometry
DOI	10.1039/c6ja00433d
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Cu MS Blood plasma Metabolism Composite material Isotope Alkali metal Ion Ion-exchange resin The Matrix Confidence interval Diurnal cycle
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Analytical Chemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

High-Precision Isotopic Analysis of Cu and Fe via Multi-Collector Inductively Coupled Plasma-Mass Spectrometry Reveals Lipopolysaccharide-Induced Inflammatory Effects in Blood Plasma and Brain Tissues

Single-step separation scheme and high-precision isotopic ratios analysis of Sr–Nd–Hf in silicate materials

Environmental monitoring of plutonium at ultratrace level in natural water (Sea of Galilee—Israel) by ICP-SFMS and MC-ICP-MS

Multi-collector icp-ms analysis of pb isotope ratios in rocks: data, procedure and caution.

Determination of Boron Isotope Ratios in Tooth Enamel by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) After Matrix Separation by Ion Exchange Chromatography

High Precision Zinc Stable Isotope Measurement of Certified Biological Reference Materials Using the Double Spike Technique and Multiple Collector-ICP-MS

State-of-the-art and progress in precise and accurate isotope ratio measurements by icp-ms and la-icp-ms{ plenary lecture (2002).

Alzheimer's Disease and Age-Related Changes in the Cu Isotopic Composition of Blood Plasma and Brain Tissues of the APP^NL-G-F Murine Model Revealed by Multi-Collector ICP-Mass Spectrometry.

Sources of mass bias and isotope ratio variation in multi-collector ICP-MS: optimization of instrumental parameters based on experimental observations

High-precision isotopic analysis of Cu in blood serum via multi-collector ICP-mass spectrometry for clinical investigation: steps towards improved robustness and higher sample throughput

Similar Documents

High-Precision Isotopic Analysis of Cu and Fe via Multi-Collector Inductively Coupled Plasma-Mass Spectrometry Reveals Lipopolysaccharide-Induced Inflammatory Effects in Blood Plasma and Brain Tissues

Single-step separation scheme and high-precision isotopic ratios analysis of Sr–Nd–Hf in silicate materials

Environmental monitoring of plutonium at ultratrace level in natural water (Sea of Galilee—Israel) by ICP-SFMS and MC-ICP-MS

Multi-collector icp-ms analysis of pb isotope ratios in rocks: data, procedure and caution.

Determination of Boron Isotope Ratios in Tooth Enamel by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) After Matrix Separation by Ion Exchange Chromatography

High Precision Zinc Stable Isotope Measurement of Certified Biological Reference Materials Using the Double Spike Technique and Multiple Collector-ICP-MS

State-of-the-art and progress in precise and accurate isotope ratio measurements by icp-ms and la-icp-ms{ plenary lecture (2002).

Alzheimer's Disease and Age-Related Changes in the Cu Isotopic Composition of Blood Plasma and Brain Tissues of the APPNL-G-F Murine Model Revealed by Multi-Collector ICP-Mass Spectrometry.

Sources of mass bias and isotope ratio variation in multi-collector ICP-MS: optimization of instrumental parameters based on experimental observations

High-precision isotopic analysis of Cu in blood serum via multi-collector ICP-mass spectrometry for clinical investigation: steps towards improved robustness and higher sample throughput

Alzheimer's Disease and Age-Related Changes in the Cu Isotopic Composition of Blood Plasma and Brain Tissues of the APP^NL-G-F Murine Model Revealed by Multi-Collector ICP-Mass Spectrometry.