NDLI: DNA/protein binding, DNA cleavage, cytotoxicity, superoxide radical scavenging and molecular docking studies of copper(ii) complexes containing N-benzyl-N′-aryl-N′′-benzoylguanidine ligands

Please wait, while we are loading the content...

DNA/protein binding, DNA cleavage, cytotoxicity, superoxide radical scavenging and molecular docking studies of copper(ii) complexes containing N-benzyl-N′-aryl-N′′-benzoylguanidine ligands

Back cover

Inorganic Chemistry Frontiers Vol - 2 Issue - 7

Inorganic Chemistry Frontiers Vol - 2 Issue - 6

Inorganic Chemistry Frontiers Vol - 2 Issue - 5

Inorganic Chemistry Frontiers Vol - 2 Issue - 4

Inorganic Chemistry Frontiers Vol - 2 Issue - 3

Inorganic Chemistry Frontiers Vol - 2 Issue - 2

Inorganic Chemistry Frontiers Vol -1

DNA/protein binding, DNA cleavage, cytotoxicity, superoxide radical scavenging and molecular docking studies of copper(ii) complexes containing N-benzyl-N′-aryl-N′′-benzoylguanidine ligands

Content Provider	Royal Society of Chemistry (RSC)
Author	Perumal, Paramasivan Thirumalai Karvembu, Ramasamy Arun, Yuvaraj Bhuvanesh, Nattamai S. P. Sreekanth, Anandaram Jeyalakshmi, Kumaramangalam
Copyright Year	2015
Abstract	Five copper(II) complexes containing N,N′,N′′-trisubstituted guanidine ligands were synthesized and characterized by elemental analyses and UV-Visible, FT-IR, EPR and mass spectroscopic techniques. The synthesized copper(II) complexes (1–5) bear the general formula [Cu{C6H5CONC(NR)NHCH2C6H5}2] where R = phenyl (1), 4-methylphenyl (2), 4-ethoxyphenyl (3), 2-methoxyphenyl (4) or 1-naphthyl (5). The four coordinated square planar geometry of the complexes was confirmed by the single crystal X-ray diffraction study. The interaction of the Cu(II) complexes with calf thymus DNA (CT DNA) was explored using absorption and fluorescence spectroscopic methods. The results revealed that the complexes have an affinity constant for DNA in the order of 104 M−1 and the mode of interaction is non-covalent intercalation. The DNA cleavage study showed that the complexes cleaved DNA without any external agent. The interaction of Cu(II) complexes with bovine serum albumin (BSA) was also studied using absorption and fluorescence techniques. The cytotoxic activity of the Cu(II) complexes was probed in vitro against human breast (MCF7) and lung (A549) cancer cell lines. The complexes were also tested against mouse embryonic fibroblast (NIH 3T3) cell lines. Complexes 1 and 3 have good cytotoxic activity which is comparable with the cyclophosphamide drug. The complexes were less cytotoxic towards normal cell lines showing that they affect only cancer cell lines. Superoxide radical scavenging property of the complexes was assessed using the NBT assay. Copper(II) complexes showed appreciable superoxide radical scavenging activity with IC50 values ranging from 1.53 to 5.62 μM. A further molecular docking technique was employed to understand the binding of the complexes toward the molecular target DNA and human DNA topoisomerase I.
Starting Page	780
Ending Page	798
Page Count	19
File Format	HTM / HTML PDF
ISSN	20521553
Volume Number	2
Issue Number	8
Journal	Inorganic Chemistry Frontiers
DOI	10.1039/c4qi00234b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	EPR BSA NIH NBT Copper Guanidine Elemental analysis Fourier-transform infrared spectroscopy Phenyl group Molecular geometry X-ray crystallography Thymus DNA Fluorescence Bovine serum albumin Cytotoxicity MCF-7 Lung Cancer Fibroblast National Institutes of Health Cyclophosphamide Nitro blue tetrazolium chloride Superoxide IC50 Docking (molecular) Topoisomerase
Content Type	Text
Resource Type	Article
Subject	Inorganic Chemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A Gain of Superoxide Dismutase (SOD) Activity Obtained with CCS, the Copper Metallochaperone for SOD1

Novel mitochondria targeted copper(ii) complexes of ferrocenyl terpyridine and anticancer active 8-hydroxyquinolines showing remarkable cytotoxicity, DNA and protein binding affinity

Reaction of the Desulfoferrodoxin from Desulfoarculus baarsii with Superoxide Anion EVIDENCE FOR A SUPEROXIDE REDUCTASE ACTIVITY

Copper Transport and Metabolism Are Normal in Aceruloplasminemic Mice

Synthesis of novel heterobimetallic copper(i) hydrazone Schiff base complexes: A comparative study on the effect of heterocyclic hydrazides towards interaction with DNA/protein, free radical scavenging and cytotoxicity

Copper Activation of Superoxide Dismutase 1 (SOD1) in Vivo ROLE FOR PROTEIN-PROTEIN INTERACTIONS WITH THE COPPER CHAPERONE FOR SOD1

Solvent-Free Synthesis, DNA-Topoisomerase II Activity and Molecular Docking Study of New Asymmetrically N,N'-Substituted Ureas

Activation of Matrix Metalloproteinase-2 by Overexpression of Manganese Superoxide Dismutase in Human Breast Cancer MCF-7 Cells Involves Reactive Oxygen Species

A Copper-responsive Transcription Factor, CRF1, Mediates Copper and Cadmium Resistance in Yarrowia lipolytica

DNA/protein binding, DNA cleavage, cytotoxicity, superoxide radical scavenging and molecular docking studies of copper(ii) complexes containing N-benzyl-N′-aryl-N′′-benzoylguanidine ligands

Similar Documents

A Gain of Superoxide Dismutase (SOD) Activity Obtained with CCS, the Copper Metallochaperone for SOD1

Novel mitochondria targeted copper(ii) complexes of ferrocenyl terpyridine and anticancer active 8-hydroxyquinolines showing remarkable cytotoxicity, DNA and protein binding affinity

Reaction of the Desulfoferrodoxin from Desulfoarculus baarsii with Superoxide Anion EVIDENCE FOR A SUPEROXIDE REDUCTASE ACTIVITY

Copper Transport and Metabolism Are Normal in Aceruloplasminemic Mice

Synthesis of novel heterobimetallic copper(i) hydrazone Schiff base complexes: A comparative study on the effect of heterocyclic hydrazides towards interaction with DNA/protein, free radical scavenging and cytotoxicity

Copper Activation of Superoxide Dismutase 1 (SOD1) in Vivo ROLE FOR PROTEIN-PROTEIN INTERACTIONS WITH THE COPPER CHAPERONE FOR SOD1

Solvent-Free Synthesis, DNA-Topoisomerase II Activity and Molecular Docking Study of New Asymmetrically N,N'-Substituted Ureas

Activation of Matrix Metalloproteinase-2 by Overexpression of Manganese Superoxide Dismutase in Human Breast Cancer MCF-7 Cells Involves Reactive Oxygen Species

A Copper-responsive Transcription Factor, CRF1, Mediates Copper and Cadmium Resistance in Yarrowia lipolytica

DNA/protein binding, DNA cleavage, cytotoxicity, superoxide radical scavenging and molecular docking studies of copper(ii) complexes containing N-benzyl-N′-aryl-N′′-benzoylguanidine ligands