NDLI: Matrix stiffness exerts biphasic control over monocyte–endothelial adhesion <i>via</i> Rho-mediated ICAM-1 clustering

Please wait, while we are loading the content...

PDMS microwells for multi-parametric monitoring of single mitochondria on a large scale: a study of their individual membrane potential and endogenous NADH

Dynamic transcription factor activity networks in response to independently altered mechanical and adhesive microenvironmental cues

An in vitro–in silico interface platform for spatiotemporal analysis of pattern formation in collective epithelial cells

Matrix stiffness exerts biphasic control over monocyte–endothelial adhesion via Rho-mediated ICAM-1 clustering

Mathematical modelling of microtumour infiltration based on in vitro experiments

Investigating the mechanical properties of zona pellucida of whole human oocytes by atomic force spectroscopy

Quantitative analysis of B-lymphocyte migration directed by CXCL13

Inside back cover

Back cover

Integrative Biology Vol - 8 Issue - 7

Integrative Biology Vol - 8 Issue - 6

Integrative Biology Vol - 8 Issue - 5

Integrative Biology Vol - 8 Issue - 4

Integrative Biology Vol - 8 Issue - 3

Integrative Biology Vol - 8 Issue - 2

Integrative Biology Vol -7

Integrative Biology Vol -6

Integrative Biology Vol -5

Integrative Biology Vol -4

Integrative Biology Vol -3

Integrative Biology Vol -2

Integrative Biology Vol -1

Matrix stiffness exerts biphasic control over monocyte–endothelial adhesion via Rho-mediated ICAM-1 clustering

Content Provider	Royal Society of Chemistry (RSC)
Author	Ghosh, Kaustabh Quach, Boi Messaoudi-Powers, Ilhem Scott, Harry A. Ardekani, Soroush Yang, Xiao Cabrera, Andrea P. Wilson, Randall
Copyright Year	2016
Abstract	Leukocyte–endothelial adhesion is a critical early step in chronic vascular inflammation associated with diabetes, emphysema, and aging. Importantly, these conditions are also marked by abnormal subendothelial matrix crosslinking (stiffness). Yet, whether and how abnormal matrix stiffness contributes to leukocyte–endothelial adhesion remains poorly understood. Using a co-culture of human monocytic cells and human microvascular endothelial cells (ECs) grown on matrices of tunable stiffness, we demonstrate that matrix stiffness exerts biphasic control over monocyte–EC adhesion, with both matrix softening and stiffening eliciting a two-fold increase in this adhesive interaction. This preferential endothelial adhesivity on softer and stiffer matrices was consistent with a significant increase in α-actinin-4-associated endothelial ICAM-1 clustering, a key determinant of monocyte–EC adhesion. Further, the enhanced ICAM-1 clustering on soft and stiff matrices correlated strongly with an increase in Rho activity and ROCK2 expression. Importantly, inhibition of Rho/ROCK activity blocked the effects of abnormal matrix stiffness on ICAM-1 clustering and monocyte–EC adhesion. Thus, these findings implicate matrix stiffness-dependent ICAM-1 clustering as an important regulator of vascular inflammation and provide the rationale for closely examining mechanotransduction pathways as new molecular targets for anti-inflammatory therapy.
Starting Page	869
Ending Page	878
Page Count	10
File Format	HTM / HTML PDF
ISSN	17579694
Volume Number	8
Issue Number	8
Journal	Integrative Biology
DOI	10.1039/c6ib00084c
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	EC Inflammation Diabetes mellitus Chronic obstructive pulmonary disease Extracellular matrix Stiffness Endothelium Actinin ICAM-1 Determinant Rho family of GTPases ROCK2 Mechanotransduction
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Biophysics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Adhesion signaling – crosstalk between integrins, Src and Rho

Global architecture of the F-actin cytoskeleton regulates cell shape-dependent endothelial mechanotransduction

Circulating galectin-3 promotes tumor-endothelium-adhesion by upregulating ICAM-1 in endothelium-derived extracellular vesicles

The assembly of integrin adhesion complexes requires both extracellular matrix and intracellular rho/rac GTPases (1995)

The assembly of integrin adhesion complexes require both extracellular matrix and intracellular Rho/Rac GTPases (1995)

Monocyte adhesion and spreading on human endothelial cells is dependent on Rho-regulated receptor clustering (1999)

Monocyte adhesion and spreading on human endothelial cells is dependent on Rho-regulated receptor clustering (1999)

Ripasudil Attenuates Lipopolysaccharide (LPS)-Mediated Apoptosis and Inflammation in Pulmonary Microvascular Endothelial Cells via ROCK2/eNOS Signaling.

Hyperketonemia increases monocyte adhesion to endothelial cells and is mediated by LFA-1 expression in monocytes and ICAM-1 expression in endothelial cells.

Matrix stiffness exerts biphasic control over monocyte–endothelial adhesion via Rho-mediated ICAM-1 clustering

Similar Documents

Adhesion signaling – crosstalk between integrins, Src and Rho

Global architecture of the F-actin cytoskeleton regulates cell shape-dependent endothelial mechanotransduction

Circulating galectin-3 promotes tumor-endothelium-adhesion by upregulating ICAM-1 in endothelium-derived extracellular vesicles

The assembly of integrin adhesion complexes requires both extracellular matrix and intracellular rho/rac GTPases (1995)

The assembly of integrin adhesion complexes require both extracellular matrix and intracellular Rho/Rac GTPases (1995)

Monocyte adhesion and spreading on human endothelial cells is dependent on Rho-regulated receptor clustering (1999)

Monocyte adhesion and spreading on human endothelial cells is dependent on Rho-regulated receptor clustering (1999)

Ripasudil Attenuates Lipopolysaccharide (LPS)-Mediated Apoptosis and Inflammation in Pulmonary Microvascular Endothelial Cells via ROCK2/eNOS Signaling.

Hyperketonemia increases monocyte adhesion to endothelial cells and is mediated by LFA-1 expression in monocytes and ICAM-1 expression in endothelial cells.

Matrix stiffness exerts biphasic control over monocyte–endothelial adhesion via Rho-mediated ICAM-1 clustering