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| Content Provider | Royal Society of Chemistry (RSC) |
|---|---|
| Author | Kumar, Supriya K. Kim, Myung Hee Shirahama, Hitomi Cho, Nam-Joon Lee, Jae Ho Seo, Jeongeun |
| Copyright Year | 2016 |
| Abstract | Loss of function is a major challenge for hepatocytes that are cultured on two-dimensional (2D) cell culture platforms. Biofunctionalized three-dimensional (3D) scaffolds produced by microfabrication strategies can overcome these limitations by presenting vital environmental cues, strong mechanical properties, and three-dimensional geometry to enable high-fidelity liver tissue engineering. Herein, we report the detailed investigation of hepatocarcinoma (Huh 7.5) cellular behavior in a collagen-functionalized microsphere-templated poly(ethylene glycol) (PEG) hydrogel scaffold which promotes 3D hepatic sheet morphology. Collagen conjugation led to improved liver-specific functions, including albumin production and cytochrome P450 (CYP450) activity. Importantly, the gene expression of numerous cell-adhesion markers was enhanced along with stimulated innate hepatocyte fibronectin production. Taken together, the findings reveal a close connection between hepatic cell morphology and gene expression, offering evidence that surface-coated collagen in the 3D hydrogel platform triggers the upregulation of hepatocyte-specific transcription factors and the secretion of liver metabolic markers. |
| Starting Page | 156 |
| Ending Page | 166 |
| Page Count | 11 |
| File Format | HTM / HTML PDF |
| ISSN | 17579694 |
| Volume Number | 8 |
| Issue Number | 2 |
| Journal | Integrative Biology |
| DOI | 10.1039/c5ib00269a |
| Language | English |
| Publisher | Royal Society of Chemistry |
| Access Restriction | Open |
| Subject Keyword | Hepatocyte Cell culture Three-dimensional space Microfabrication Geometry Liver Tissue engineering Hepatocellular carcinoma Ethylene glycol Gel Albumin Cytochrome P450 Gene Fibronectin Collagen |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Biochemistry Biophysics |
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