NDLI: On being the right size: scaling effects in designing a human-on-a-chip

Please wait, while we are loading the content...

Microfluidic technique to measure intratumoral transport and calculate drug efficacy shows that binding is essential for doxorubicin and release hampers Doxil

Back cover

Integrative Biology Vol - 5 Issue - 8

Integrative Biology Vol - 5 Issue - 7

Integrative Biology Vol - 5 Issue - 6

Integrative Biology Vol - 5 Issue - 5

Integrative Biology Vol - 5 Issue - 4

Integrative Biology Vol - 5 Issue - 3

Integrative Biology Vol - 5 Issue - 2

Integrative Biology Vol -4

Integrative Biology Vol -3

Integrative Biology Vol -2

Integrative Biology Vol -1

On being the right size: scaling effects in designing a human-on-a-chip

Content Provider	Royal Society of Chemistry (RSC)
Author	Inoue, Mayumi Chun, Tae-Hwa Takayama, Shuichi Labuz, Joseph M. Leung, Brendan M. Moraes, Christopher
Copyright Year	2013
Abstract	Developing a human-on-a-chip by connecting multiple model organ systems would provide an intermediate screen for therapeutic efficacy and toxic side effects of drugs prior to conducting expensive clinical trials. However, correctly designing individual organs and scaling them relative to each other to make a functional microscale human analog is challenging, and a generalized approach has yet to be identified. In this work, we demonstrate the importance of rational design of both the individual organ and its relationship with other organs, using a simple two-compartment system simulating insulin-dependent glucose uptake in adipose tissues. We demonstrate that inter-organ scaling laws depend on both the number of cells and the spatial arrangement of those cells within the microfabricated construct. We then propose a simple and novel inter-organ ‘metabolically supported functional scaling’ approach predicated on maintaining in vivo cellular basal metabolic rates by limiting resources available to cells on the chip. This approach leverages findings from allometric scaling models in mammals that limited resources in vivo prompt cells to behave differently than in resource-rich in vitro cultures. Although applying scaling laws directly to tissues can result in systems that would be quite challenging to implement, engineering workarounds may be used to circumvent these scaling issues. Specific workarounds discussed include the limited oxygen carrying capacity of cell culture media when used as a blood substitute and the ability to engineer non-physiological structures to augment organ function, to create the transport-accessible, yet resource-limited environment necessary for cells to mimic in vivo functionality. Furthermore, designing the structure of individual tissues in each organ compartment may be a useful strategy to bypass scaling concerns at the inter-organ level.
Starting Page	1149
Ending Page	1161
Page Count	13
File Format	HTM / HTML PDF
ISSN	17579694
Volume Number	5
Issue Number	9
Journal	Integrative Biology
DOI	10.1039/c3ib40040a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Glucose Adipocyte Microfabrication Allometry Oxygen Cell culture Blood substitute
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Biophysics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

On being the right size: scaling effects in designing a human-on-a-chip

Effect of cell size on lipid synthesis by human adipose tissue in vitro

A 3D Cell Culture Organ-on-a-Chip Platform With a Breathable Hemoglobin Analogue Augments and Extends Primary Human Hepatocyte Functions in vitro

Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment

Effect of cell size on lipolysis and antilipolytic action of insulin in human fat cells

The Use of Polycondensed Hemoglobin as the Basis of a Blood Substitute Capable of Transporting Oxygen

A screen for short-range paracrine interactions

Organs-on-chips: breaking the in vitro impasse

Integration column: microwell arrays for mammalian cell culture

On being the right size: scaling effects in designing a human-on-a-chip

Similar Documents

On being the right size: scaling effects in designing a human-on-a-chip

Effect of cell size on lipid synthesis by human adipose tissue in vitro

A 3D Cell Culture Organ-on-a-Chip Platform With a Breathable Hemoglobin Analogue Augments and Extends Primary Human Hepatocyte Functions in vitro

Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment

Effect of cell size on lipolysis and antilipolytic action of insulin in human fat cells

The Use of Polycondensed Hemoglobin as the Basis of a Blood Substitute Capable of Transporting Oxygen

A screen for short-range paracrine interactions

Organs-on-chips: breaking the in vitro impasse

Integration column: microwell arrays for mammalian cell culture

On being the right size: scaling effects in designing a human-on-a-chip