NDLI: Molecular mechanisms of biomaterial-driven osteogenic differentiation in human mesenchymal stromal cells

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Molecular mechanisms of biomaterial-driven osteogenic differentiation in human mesenchymal stromal cells

Content Provider	Royal Society of Chemistry (RSC)
Author	Birgani, Zeinab Tahmasebi Monticone, Veronica Blitterswijk, Clemens Danoux, Charlène Reinders, Marcel Barradas, Ana M. C. Groot, Florence Barrère-de Yuan, Huipin Hulsman, Marc Boer, Jan de Fernandes, Hugo Habibovic, Pamela
Copyright Year	2013
Abstract	Calcium phosphate (CaP) based ceramics are used as bone graft substitutes in the treatment of bone defects. The physico-chemical properties of these materials determine their bioactivity, meaning that molecular and cellular responses in the body will be tuned accordingly. In a previous study, we compared two porous CaP ceramics, hydroxyapatite (HA) and β-tricalcium phosphate (TCP), which, among other properties, differ in their degradation behaviour in vitro and in vivo, and we demonstrated that the more degradable β-TCP induced more bone formation in a heterotopic model in sheep. This is correlated to in vitro data, where human bone marrow derived mesenchymal stromal cells (MSC) exhibited higher expression of osteogenic differentiation markers, such as osteopontin, osteocalcin and bone sialoprotein, when cultured in β-TCP than in HA. More recently, we also showed that this effect could be mimicked in vitro by exposure of MSC to high concentrations of calcium ions (Ca2+). To further correlate surface physico-chemical dynamics of HA and β-TCP ceramics with the molecular response of MSC, we followed Ca2+ release and surface changes in time as well as cell attachment and osteogenic differentiation of MSC on these ceramics. Within 24 hours, we observed differences in cell morphology, with MSC cultured in β-TCP displaying more pronounced attachment and spreading than cells cultured in HA. In the same time frame, β-TCP induced expression of G-protein coupled receptor (GPCR) 5A and regulator of G-protein signaling 2, revealed by DNA microarray analysis. These genes, associated with the protein kinase A and GPCR signaling pathways, may herald the earliest response of MSC to bone-inducing ceramics.
Starting Page	920
Ending Page	931
Page Count	12
File Format	HTM / HTML PDF
ISSN	17579694
Volume Number	5
Issue Number	7
Journal	Integrative Biology
DOI	10.1039/c3ib40027a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	TCP MSC HA Calcium phosphate Bone grafting Bone Hydroxylapatite Phosphorylation Tenocyclidine Bone marrow Osteopontin Osteocalcin Bone sialoprotein Calcium G protein\u2013coupled receptor DNA microarray Protein kinase A
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Biophysics

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