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From single cells to biology

A single cell bioengineering approach to elucidate mechanisms of adult stem cell self-renewal

Intracellular protein trafficking kinetics in chronic myeloid leukemia stem cells using a microfluidic platform

Single-cell level co-culture platform for intercellular communication

Quantitative and dynamic assay of single cell chemotaxis

High-throughput clonal analysis of neural stem cells in microarrayed artificial niches

Migration dynamics of breast cancer cells in a tunable 3D interstitial flow chamber

The mechanical memory of lung myofibroblasts

Hyaluronic acid matrices show matrix stiffness in 2D and 3D dictates cytoskeletal order and myosin-II phosphorylation within stem cells

Quantification of local matrix deformations and mechanical properties during capillary morphogenesis in 3D

Growth of lung cancer cells in three-dimensional microenvironments reveals key features of tumor malignancy

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Quantification of local matrix deformations and mechanical properties during capillary morphogenesis in 3D

Content Provider	Royal Society of Chemistry (RSC)
Author	Weidling, John W. Digman, Michelle A. Kniazeva, Ekaterina Botvinick, Elliot L. Gratton, Enrico Putnam, Andrew J. Singh, Rahul
Copyright Year	2012
Abstract	Reciprocal mechanical interactions between cells and the extracellular matrix (ECM) are thought to play important instructive roles in branching morphogenesis. However, most studies to date have failed to characterize these interactions on a length scale relevant to cells, especially in three-dimensional (3D) matrices. Here we utilized two complementary methods, spatio-temporal image correlation spectroscopy (STICS) and laser optical tweezers-based active microrheology (AMR), to quantify endothelial cell (EC)-mediated deformations of individual ECM elements and the local ECM mechanical properties, respectively, during the process of capillary morphogenesis in a 3D cell culture model. In experiments in which the ECM density was systematically varied, STICS revealed that the rate at which ECs deformed individual ECM fibers on the microscale positively correlated with capillary sprouting on the macroscale. ECs expressing constitutively active V14-RhoA displaced individual matrix fibers at significantly faster rates and displayed enhanced capillary sprouting relative to wild-type cells, while those expressing dominant-negative N19-RhoA behaved in an opposite fashion. In parallel, AMR revealed a local stiffening of the ECM proximal to the tips of sprouting ECs. By quantifying the dynamic physical properties of the cell-ECM interface in both space and time, we identified a correlation linking ECM deformation rates and local ECM stiffening at the microscale with capillary morphogenesis at the macroscale.
Starting Page	431
Ending Page	439
Page Count	9
File Format	HTM / HTML PDF
ISSN	17579694
Volume Number	4
Issue Number	4
Journal	Integrative Biology
DOI	10.1039/c2ib00120a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	AMR EC Extracellular matrix Morphogenesis Three-dimensional space Two-dimensional nuclear magnetic resonance spectroscopy Laser Microrheology Endothelium 3D cell culture RHOA N postcode area
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Biophysics

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Quantification of Local Matrix Mechanical Properties and Deformations During Capillary Morphogenesis in 3D

Quantification of local matrix deformations and mechanical properties during capillary morphogenesis in 3D† ‡

Endothelial cell dynamics during anastomosis in vitro

Engineering strategies to recapitulate epithelial morphogenesis within synthetic three-dimensional extracellular matrix with tunable mechanical properties

Endogenous patterns of mechanical stress are required for branching morphogenesis

Three-Dimensional Modeling of Mechanical Forces in the Extracellular Matrix during Epithelial Lumen Formation

Design, fabrication and implementation of a novel multi-parameter control microfluidic platform for three-dimensional cell culture and real-time imaging

Matritecture: Mapping the extracellular matrix architecture during health and disease.

The effect of matrix density on the regulation of 3-d capillary morphogenesis.

Quantification of local matrix deformations and mechanical properties during capillary morphogenesis in 3D

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Quantification of Local Matrix Mechanical Properties and Deformations During Capillary Morphogenesis in 3D

Quantification of local matrix deformations and mechanical properties during capillary morphogenesis in 3D† ‡

Endothelial cell dynamics during anastomosis in vitro

Engineering strategies to recapitulate epithelial morphogenesis within synthetic three-dimensional extracellular matrix with tunable mechanical properties

Endogenous patterns of mechanical stress are required for branching morphogenesis

Three-Dimensional Modeling of Mechanical Forces in the Extracellular Matrix during Epithelial Lumen Formation

Design, fabrication and implementation of a novel multi-parameter control microfluidic platform for three-dimensional cell culture and real-time imaging

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The effect of matrix density on the regulation of 3-d capillary morphogenesis.

Quantification of local matrix deformations and mechanical properties during capillary morphogenesis in 3D