NDLI: MudPIT analysis of released proteins in <i>Pseudomonas aeruginosa</i> laboratory and clinical strains in relation to pro-inflammatory effects

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Contents list

Emerging methodologies to investigate lipid–protein interactions

Epithelial cell guidance by self-generated EGF gradients

MudPIT analysis of released proteins in Pseudomonas aeruginosa laboratory and clinical strains in relation to pro-inflammatory effects

Quantifying cellular differentiation by physical phenotype using digital holographic microscopy

A progressive approach on zebrafish toward sensitive evaluation of nanoparticles' toxicity

Statistical platform to discern spatial and temporal coordination of endothelial sprouting

CdTe/CdSe quantum dots improve the binding affinities between α-amylase and polyphenols

A framework for designing and analyzing binary decision-making strategies in cellular systems

Combinatorial cell–3D biomaterials cytocompatibility screening for tissue engineering using bioinspired superhydrophobic substrates

Cell cycle synchronization by nutrient modulation

Calibrating spatio-temporal models of leukocyte dynamics against in vivo live-imaging data using approximate Bayesian computation

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Integrative Biology Vol - 4 Issue - 2

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Integrative Biology Vol -2

Integrative Biology Vol -1

MudPIT analysis of released proteins in Pseudomonas aeruginosa laboratory and clinical strains in relation to pro-inflammatory effects

Content Provider	Royal Society of Chemistry (RSC)
Author	Döring, Gerd Sorio, Claudio Palma, Antonella De Assael, Baroukh Maurice Mauri, Pierluigi Bergamini, Gabriella Bragonzi, Alessandra Melotti, Paola Silvestre, Dario Di Benazzi, Louise Cigana, Cristina
Copyright Year	2012
Abstract	Pseudomonas aeruginosa (Pa) is the most common virulent pathogen contributing to the pathogenesis of cystic fibrosis (CF). During bacterial lung colonization, the products of its metabolism are released in the extracellular space contributing to the pathogenic events associated with its presence. To gain insights on the mechanisms involved in the Pa pathogenesis we focused our attention on proteins released by Pa using a MudPIT approach combined with cell biology assays. Conditioned medium (CM) collected under aerobic and microaerobic conditions from Pa clinical strains (in early and late colonization), unlike the laboratory strain, induced expression of IL-8 mRNA in CF airway epithelial cells. We have identified proteins released by clinically relevant Pa strains, focusing on the pro-inflammatory effects as metalloproteases (MMPs). In fact, their expression pattern was associated with the highest pro-inflammatory activity measured in the early clinically isolated strain. The relation was further supported by the result of the analysis of a larger and independent set of Pa isolates derived from sporadically and chronically infected CF patients: 76% of sporadic samples expressed protease activity (n = 44), while only 27% scored positive in the chronically infected individuals (n = 38, p < 0.0001, Fisher's exact test). Finally, looking for a possible mechanism of action of bacterial MMPs, we found that CM from early clinical isolates can cleave CXCR1 on the surface of human neutrophils, suggesting a potential role for the bacterially released MMPs in the protection of the pathogen from the host's response.
Starting Page	270
Ending Page	279
Page Count	10
File Format	HTM / HTML PDF
ISSN	17579694
Volume Number	4
Issue Number	3
Journal	Integrative Biology
DOI	10.1039/c2ib00127f
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Pa Pseudomonas aeruginosa Fisher Pathogen Fibrosis Advertisement film Lung Metabolism Mudpit (TV series) Molecular biology Aerobic organism Interleukin 8 Messenger RNA Independent set (graph theory) Protease Interleukin 8 receptor alpha Neutrophil
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Biophysics

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Sputum and Plasma Neutrophil Elastase in Stable Adult Patients With Cystic Fibrosis in Relation to Chronic Pseudomonas Aeruginosa Colonization.

Nitrite reductase from Pseudomonas aeruginosa released by antimicrobial agents and complement induces interleukin-8 production in bronchial epithelial cells.

Interactions between Neutrophils and Pseudomonas aeruginosa in Cystic Fibrosis

Vitamin D inhibits pro-inflammatory cytokines in the airways of cystic fibrosis patients infected by Pseudomonas aeruginosa- pilot study

IL-33 is expressed in epithelia from patients with cystic fibrosis and potentiates neutrophil recruitment.

Nonclassical pathway of pseudomonas aeruginosa dna-induced interleukin-8 secretion in cystic fibrosis airway epithelial cells (2005).

Antibodies Against Pseudomonas aeruginosa Alkaline Protease Directly Enhance Disruption of Neutrophil Extracellular Traps Mediated by This Enzyme

Nonclassical pathway of Pseudomonas aeruginosa DNA-induced interleukin-8 secretion in cystic fibrosis airway epithelial cells.

Released upon cell lysis during stationary phase is essential for aerobic survival of a pseudomonas aeruginosa oxyr mutant at (2000).

MudPIT analysis of released proteins in Pseudomonas aeruginosa laboratory and clinical strains in relation to pro-inflammatory effects

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Sputum and Plasma Neutrophil Elastase in Stable Adult Patients With Cystic Fibrosis in Relation to Chronic Pseudomonas Aeruginosa Colonization.

Nitrite reductase from Pseudomonas aeruginosa released by antimicrobial agents and complement induces interleukin-8 production in bronchial epithelial cells.

Interactions between Neutrophils and Pseudomonas aeruginosa in Cystic Fibrosis

Vitamin D inhibits pro-inflammatory cytokines in the airways of cystic fibrosis patients infected by Pseudomonas aeruginosa- pilot study

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Released upon cell lysis during stationary phase is essential for aerobic survival of a pseudomonas aeruginosa oxyr mutant at (2000).

MudPIT analysis of released proteins in Pseudomonas aeruginosa laboratory and clinical strains in relation to pro-inflammatory effects