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Integrative Biology Vol -9

Integrative Biology Vol -8

Integrative Biology Vol -7

Integrative Biology Vol -6

Integrative Biology Vol -5

Integrative Biology Vol -4

Integrative Biology Vol -3

Integrative Biology Vol -2

Integrative Biology Vol - 2 Issue - 11-12

Front cover

Contents

Inside front cover

Introduction to the Mechanisms of Directed Cell Migration themed issue

Tools for analyzing cell shape changes during chemotaxis

Determinants of leader cells in collective cell migration

Critical review: cellular mechanobiology and amoeboid migration

Biological applications of microfluidic gradient devices

Microfluidics for bacterial chemotaxis

An arrayed high-content chemotaxis assay for patient diagnosis

Directional decisions during neutrophil chemotaxis inside bifurcating channels

Open access microfluidic device for the study of cell migration during chemotaxis

Gradient sensing in defined chemotactic fields

A neuron-benign microfluidic gradient generator for studying the response of mammalian neurons towards axon guidance factors

Microfluidic platform for chemotaxis in gradients formed by CXCL12 source-sink cells

Use of the parameterised finite element method to robustly and efficiently evolve the edge of a moving cell

A new chemotaxis device for cell migration studies

Back matter

Back cover

Integrative Biology Vol - 2 Issue - 9

Integrative Biology Vol - 2 Issue - 7-8

Integrative Biology Vol - 2 Issue - 5-6

Integrative Biology Vol - 2 Issue - 4

Integrative Biology Vol - 2 Issue - 2-3

Integrative Biology Vol -1

An arrayed high-content chemotaxis assay for patient diagnosis

Content Provider	Royal Society of Chemistry (RSC)
Author	Huttenlocher, Anna Verbsky, James Beebe, David Berthier, Erwin Surfus, Jill
Copyright Year	2010
Abstract	Chemotaxis assays are essential tools for the study of gradient sensing and directed cell migration, and have the potential to aid in the diagnosis and characterization of patients with immune disorders. Current methods are limited in their ability to meet the more demanding requirements for clinical applications. Because patient samples have a short lifespan and sometimes a limited volume (e.g. pediatrics), the operational requirements for an efficient chemotaxis assay are increased in the clinical setting. Here we describe a microscale assay platform for gradient generation that overcomes these limitations. Passive fluidic methods are leveraged to provide a reliable microfluidic gradient generation device, operable in only three pipetting steps. In addition, arrayed imaging and advanced cell tracking algorithms enabled a 50-fold increase in throughput over current methods. These methods were employed to aid in the diagnostic evaluation of an infant who presented with severe, recurrent bacterial infections. Analysis of the infant’s neutrophils revealed impaired cell polarization and chemotaxis in a gradient of the chemoattractant fMLP. The patient was subsequently diagnosed with an inhibitory mutation in the Rho GTPase, Rac2. The approach also enabled a microenvironmental screen of human primary neutrophil chemotaxis on fibronectin, fibrinogen and laminin with results suggesting that fibronectin, athough commonly used, may not be the most appropriate matrix protein for chemotaxis assays. Together, these findings demonstrate the use of arrayed micro-devices to aid in the diagnosis of a primary immunodeficiency disorder, and illustrate the capability for increased throughput microenvironmental studies and screening targeted to specific human diseases.
Starting Page	630
Ending Page	638
Page Count	9
File Format	HTM / HTML PDF
ISSN	17579694
Volume Number	2
Issue Number	11-12
Journal	Integrative Biology
DOI	10.1039/c0ib00030b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Chemotaxis Pediatrics Chemotaxis assay Neutrophil N-Formylmethionine-leucyl-phenylalanine Mutation Rho family of GTPases GTPase RAC2 Fibronectin Fibrinogen Laminin Extracellular matrix Protein Primary immunodeficiency
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Biophysics

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Neutrophil migration assay from a drop of blood

Stimulation of tubulin tyrosinolation in rabbit leukocytes evoked by the chemoattractant formyl-methionyl-leucyl-phenylalanine

Plenary paper Dominant negative mutation of the hematopoietic-specific Rho GTPase, Rac2, is associated with a human phagocyte immunodeficiency

Rac2, is associated with a human phagocyte immunodeficiency Dominant negative mutation of the hematopoietic-specific Rho GTPase,

Dominant negative mutation of the hematopoietic-specific Rho GTPase, Rac2, is associated with a human phagocyte immunodeficiency.

TRPC1 regulates fMLP-stimulated migration and chemotaxis of neutrophil granulocytes☆

Attachment to fibronectin or vitronectin makes human neutrophil migration sensitive to alterations in cytosolic free calcium concentration

The incorporation of fibrinogen into extracellular matrix is dependent on active assembly of a fibronectin matrix

Polymorphonuclear leukocyte migration through human amnion membrane

An arrayed high-content chemotaxis assay for patient diagnosis

Similar Documents

Neutrophil migration assay from a drop of blood

Stimulation of tubulin tyrosinolation in rabbit leukocytes evoked by the chemoattractant formyl-methionyl-leucyl-phenylalanine

Plenary paper Dominant negative mutation of the hematopoietic-specific Rho GTPase, Rac2, is associated with a human phagocyte immunodeficiency

Rac2, is associated with a human phagocyte immunodeficiency Dominant negative mutation of the hematopoietic-specific Rho GTPase,

Dominant negative mutation of the hematopoietic-specific Rho GTPase, Rac2, is associated with a human phagocyte immunodeficiency.

TRPC1 regulates fMLP-stimulated migration and chemotaxis of neutrophil granulocytes☆

Attachment to fibronectin or vitronectin makes human neutrophil migration sensitive to alterations in cytosolic free calcium concentration

The incorporation of fibrinogen into extracellular matrix is dependent on active assembly of a fibronectin matrix

Polymorphonuclear leukocyte migration through human amnion membrane

An arrayed high-content chemotaxis assay for patient diagnosis