NDLI: Characterization of the bacterial and fungal microbiome in indoor dust and outdoor air samples: a pilot study

Please wait, while we are loading the content...

Construction of a bioreporter by heterogeneously expressing a Vibrio natriegens recA::luxCDABE fusion in Escherichia coli, and genotoxicity assessments of petrochemical-contaminated groundwater in northern China

Nano-hydroxyapatite alleviates the detrimental effects of heavy metals on plant growth and soil microbes in e-waste-contaminated soil

Correction: Impact of metallurgical activities on the content of trace elements in the spatial soil and plant parts of Rubus fruticosus L.

Back cover

Environmental Science: Processes & Impacts Vol - 18 Issue - 5

Characterization of the bacterial and fungal microbiome in indoor dust and outdoor air samples: a pilot study

Content Provider	Royal Society of Chemistry (RSC)
Author	Silverman, Frances Zhou, Yanjiao Gold, Diane R. Urch, Bruce Weinstock, George Sordillo, Joanne E. Hanson, Blake Sodergren, Erica Bautista, Eddy J. Speck, Mary Muilenberg, Michael Phipatanakul, Wanda
Copyright Year	2016
Abstract	Environmental microbes have been associated with both protective and adverse health effects in children and adults. Epidemiological studies often rely on broad biomarkers of microbial exposure (i.e. endotoxin, 1 → 3-beta-D-glucan), but fail to identify the taxonomic composition of the microbial community. Our aim was to characterize the bacterial and fungal microbiome in different types of environmental samples collected in studies of human health effects. We determined the composition of microbial communities present in home, school and outdoor air samples by amplifying and sequencing regions of rRNA genes from bacteria (16S) and fungi (18S and ITS). Samples for this pilot study included indoor settled dust (from both a Boston area birth cohort study on Home Allergens and Asthma (HAA) (n = 12) and a study of school exposures and asthma symptoms (SICAS) (n = 1)), as well as fine and coarse concentrated outdoor ambient particulate (CAP) samples (n = 9). Sequencing of amplified 16S, 18S, and ITS regions was performed on the Roche-454 Life Sciences Titanium pyrosequencing platform. Indoor dust samples were dominated by Gram-positive bacteria (Firmicutes and Actinobacteria); the most abundant bacterial genera were those related to human flora (Streptococcus, Staphylococcus, Corynebacterium and Lactobacillus). Outdoor CAPs were dominated by Gram-negative Proteobacteria from water and soil sources, in particular the genera Acidovorax, and Brevundimonas (which were present at very low levels or entirely absent in indoor dust). Phylum-level fungal distributions identified by 18S or ITS regions showed very similar findings: a predominance of Ascomycota in indoor dust and Basidiomycota in outdoor CAPs. ITS sequencing of fungal genera in indoor dust showed significant proportions of Aureobasidium and Leptosphaerulina along with some contribution from Cryptococcus, Epicoccum, Aspergillus and the human commensal Malassezia. ITS sequencing detected more than 70 fungal genera in indoor dust not observed by culture. Microbiome sequencing is feasible for different types of archived environmental samples (indoor dust, and low biomass air particulate samples), and offers the potential to study how whole communities of microbes (including unculturable taxa) influence human health.
Starting Page	713
Ending Page	724
Page Count	12
File Format	HTM / HTML PDF
ISSN	20507887
Volume Number	18
Issue Number	6
Journal	Environmental Science: Processes & Impacts
DOI	10.1039/c5em00639b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Aspergillus Boston Actinobacteria Lactobacillus Ascomycota Basidiomycota Leptosphaerulina Lipopolysaccharide Microbiota Ribosomal RNA Bacteria 16S ribosomal RNA Fungus 18S ribosomal RNA Cohort study Asthma Hoffmann-La Roche 454 Life Sciences Pyrosequencing Gram-positive bacteria Firmicutes Human microbiota Streptococcus Corynebacterium Gram-negative bacteria Proteobacteria Acidovorax Brevundimonas Cryptococcus neoformans Commensalism Malassezia Biomass
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Public Health, Environmental and Occupational Health Management, Monitoring, Policy and Law

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Placenta microbiome : Proteobacteria Firmicutes Fusobacteria Tenericutes Age 0-3 : Proteobacteria Actinobacteria > 3 years : Proteobacteria Actinobacteria Firmicutes Bacteroidetes

Detection of mutated primers and impact on targeted metagenomics results

Detection of mutated primers and impact on targeted metagenomics results

Rehmannia glutinosa reduced waist circumferences of Korean obese women possibly through modulation of gut microbiota

Molecular characterization of microbial communities in bioaerosols of a coal mine by 454 pyrosequencing and real-time PCR.

Detection of mutated primers and impact on targeted metagenomics results

Radiation-Tolerant Bacteria Isolated from High Altitude Soil in Tibet.

Intestinal microbiota of gibel carp (Carassius auratus gibelio) and its origin as revealed by 454 pyrosequencing.

Microbial analysis in primary and persistent endodontic infections by using pyrosequencing.

Characterization of the bacterial and fungal microbiome in indoor dust and outdoor air samples: a pilot study

Similar Documents

Placenta microbiome : Proteobacteria Firmicutes Fusobacteria Tenericutes Age 0-3 : Proteobacteria Actinobacteria > 3 years : Proteobacteria Actinobacteria Firmicutes Bacteroidetes

Detection of mutated primers and impact on targeted metagenomics results

Detection of mutated primers and impact on targeted metagenomics results

Rehmannia glutinosa reduced waist circumferences of Korean obese women possibly through modulation of gut microbiota

Molecular characterization of microbial communities in bioaerosols of a coal mine by 454 pyrosequencing and real-time PCR.

Detection of mutated primers and impact on targeted metagenomics results

Radiation-Tolerant Bacteria Isolated from High Altitude Soil in Tibet.

Intestinal microbiota of gibel carp (Carassius auratus gibelio) and its origin as revealed by 454 pyrosequencing.

Microbial analysis in primary and persistent endodontic infections by using pyrosequencing.

Characterization of the bacterial and fungal microbiome in indoor dust and outdoor air samples: a pilot study