NDLI: Ethanol production in syngas-fermenting <i>Clostridium ljungdahlii</i> is controlled by thermodynamics rather than by enzyme expression

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Ethanol production in syngas-fermenting Clostridium ljungdahlii is controlled by thermodynamics rather than by enzyme expression

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Ethanol production in syngas-fermenting Clostridium ljungdahlii is controlled by thermodynamics rather than by enzyme expression

Content Provider	Royal Society of Chemistry (RSC)
Author	Aristilde, L. Richter, H. Wei, H. Molitor, B. Chen, W. Angenent, L. T.
Copyright Year	2016
Abstract	Acetogenic bacteria are attracting interest as biocatalysts in the biotechnology industry, since they are able to ferment carbon monoxide (CO)-rich gases. Wild-type strains produce mainly acetate and ethanol, but genetic modifications have already broadened the product portfolio. To enhance the production of intrinsic or heterologous biochemicals, knowledge of the microbial physiology is necessary. This physiology includes two different phases: acidogenesis (growth/acetate production) and solventogenesis (starvation/ethanol production). We operated two sequential, continuous bioreactors with a pure culture of Clostridium ljungdahlii to achieve steady-state conditions in an acetate- and an ethanol-producing stage to spatially separate acidogenesis and solventogenesis. Here, nearly 2000 proteins and their differential abundance between acidogenesis and solventogenesis were identified. In addition, we measured important metabolites. The results showed that nutrient-limited conditions triggered a transition to solventogenesis without altering the differential abundance of enzymes in the central energy metabolism. Our proteomics results revealed that the enzymes for ethanol production (AOR/ADH) were consistently abundant, even during acidogenesis. Based on this work, we developed an overflow model with thermodynamic rather than genetic regulation. The model identifies acetic acid and reduced cofactors as saturation reactants. When the intracellular concentration of undissociated acetic acid reaches a thermodynamic threshold, C. ljungdahlii will be able to shunt surplus reducing equivalents toward ethanol immediately. This is important during retarded growth, when reducing equivalents can no longer be shunted toward biomass production, while the supply of CO-rich gas is still high. Nutrient availability and pH can be manipulated to achieve the desirable level of solventogenesis during bioprocessing.
Starting Page	2392
Ending Page	2399
Page Count	8
File Format	HTM / HTML PDF
ISSN	17545692
Volume Number	9
Issue Number	7
Journal	Energy & Environmental Science
DOI	10.1039/c6ee01108j
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Bacteria Biotechnology Carbon monoxide Ethanol Physiology Acidogenesis Clostridium Metabolism Proteomics Alcohol dehydrogenase Acid Cofactor (biochemistry) Biomass PH
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Pollution Renewable Energy, Sustainability and the Environment Nuclear Energy and Engineering

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Side-by-Side Comparison of Clean and Biomass-Derived, Impurity-Containing Syngas as Substrate for Acetogenic Fermentation with Clostridium ljungdahlii

Using exergy to analyse the sustainability of fermentative ethanol and acetate production from syngas via anaerobic bacteria (Clostridium ljungdahlii)

Comparison of Syngas-Fermenting Clostridia in Stirred-Tank Bioreactors and the Effects of Varying Syngas Impurities

Characterization of Clostridium ljungdahlii OTA1: a non-autotrophic hyper ethanol-producing strain

Growth and Product Formation of Clostridium ljungdahlii in Presence of Cyanide

Enhanced Ethanol Production From Carbon Monoxide by Enriched Clostridium Bacteria

Enhancement of bioethanol production in syngas fermentation with Clostridium ljungdahlii using nanoparticles.

A Narrow pH Range Supports Butanol, Hexanol, and Octanol Production from Syngas in a Continuous Co-culture of Clostridium ljungdahlii and Clostridium kluyveri with In-Line Product Extraction

Energy Conservation and Carbon Flux Distribution During Fermentation of CO or H2/CO2 by Clostridium ljungdahlii

Ethanol production in syngas-fermenting Clostridium ljungdahlii is controlled by thermodynamics rather than by enzyme expression

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Side-by-Side Comparison of Clean and Biomass-Derived, Impurity-Containing Syngas as Substrate for Acetogenic Fermentation with Clostridium ljungdahlii

Using exergy to analyse the sustainability of fermentative ethanol and acetate production from syngas via anaerobic bacteria (Clostridium ljungdahlii)

Comparison of Syngas-Fermenting Clostridia in Stirred-Tank Bioreactors and the Effects of Varying Syngas Impurities

Characterization of Clostridium ljungdahlii OTA1: a non-autotrophic hyper ethanol-producing strain

Growth and Product Formation of Clostridium ljungdahlii in Presence of Cyanide

Enhanced Ethanol Production From Carbon Monoxide by Enriched Clostridium Bacteria

Enhancement of bioethanol production in syngas fermentation with Clostridium ljungdahlii using nanoparticles.

A Narrow pH Range Supports Butanol, Hexanol, and Octanol Production from Syngas in a Continuous Co-culture of Clostridium ljungdahlii and Clostridium kluyveri with In-Line Product Extraction

Energy Conservation and Carbon Flux Distribution During Fermentation of CO or H2/CO2 by Clostridium ljungdahlii

Ethanol production in syngas-fermenting Clostridium ljungdahlii is controlled by thermodynamics rather than by enzyme expression