NDLI: NMR studies of zinc binding in a multi-histidinic peptide fragment

Please wait, while we are loading the content...

Temperature effects on the structure and dynamics of the Jahn–Teller distorted Cr²⁺ ion in aqueous solution: a hybrid QM/MM molecular dynamics simulation

A hybrid cobalt hydroxyacetate magnet: ionothermal synthesis, 3-D Co–O–Co connectivity and spin glass behavior

Markedly different cytotoxicity of the two enantiomers of C₂-symmetrical Ti(iv) phenolato complexes; mechanistic implications

One-dimensional metal-azido complex constructed by a double EO azido bridged trinuclear nickel(ii) unit: synthesis, structure and magnetic properties

Four tetrairidium carbonyl clusters linked by six diphosphino ligands: synthesis and X-ray structure of [{Ir₄(CO)₉}₄(dppmb)₆] (dppmb = 1,4-bis(diphenylphosphinomethyl)benzene

A double-stranded dinuclear cadmium(ii) helicate that assembles into chains in the solid state

Selective extradiol cleavage of catechol achieved in organized assemblies using [Fe(BPA)Cl₃] (BPA = bis(pyridylmethyl)amine)

Synthesis and complexation of heptafluoroisopropyldiphenylphosphine

Unexpected chemoselectivity in the Schiff condensation of amines with η²(C,O)- η¹(O)-coordinated aldehyde

Homogeneous photocatalytic production of hydrogen from water by a bioinspired [Fe₂S₂] catalyst with high turnover numbers

Specificity of the zinc(ii), magnesium(ii) and calcium(ii) complexation by (pyridin-2-yl)aminomethane-1,1-diphosphonic acids and related 1,3-(thiazol-2-yl) and 1,3-(benzothiazol-2-yl) derivatives

Syntheses and molecular structures of some tricobalt carbonyl clusters containing 2,4,6-trimethyl-1,3,5-trithiane

Crystallization of synthetic haemozoin (β-haematin) nucleated at the surface of lipid particles

Two new cantilever-type polyoxometalates constructed from {Mo₂O₄}²⁺ fragments and diphosphonates

Highly luminescent terpyridinyl-ethynyl functionalized dithieno[3,2-b:2′,3′-d]phospholes: synthesis, properties and complexation behavior

Two-dimensional metal–organic frameworks with blue luminescence

Disproportionation and radical formation in the coordination of “GaI” with bis(imino)pyridines

Dynamic ring-opening polymerization of silver(i) complexes with bis(amidopyridine) ligands

NMR studies of zinc binding in a multi-histidinic peptide fragment

Reactions of cisplatin with cysteine and methionine at constant pH; a computational study

Two pyridine derivatives as potential Cu(ii) and Zn(ii) chelators in therapy for Alzheimer's disease

A thioether-rich crown-based highly selective fluorescent sensor for Hg²⁺ and Ag⁺ in aqueous solution

Bis[1,4,8,11,15,18,22,25-octa(butyloxyl)phthalocyaninato] rare earth double-decker complexes: synthesis, spectroscopy, and molecular structure

Titanium ferrocenyl-phosphinimide complexes

Dissolved N₂ sensing by pH-dependent Ru complexes

Vanadium complexes having [V^IVO]²⁺ and [V^VO₂]⁺ cores with binucleating dibasic tetradentate ligands: Synthesis, characterization, catalytic and antiamoebic activities

Manganese(iii)-mediated cyclodimerization of a hydrazinyl derivative generating an unprecedented 1,2,3,5,6-substituted leuco-verdazyl ring

Investigation of para-sulfonatocalix[n]arenes [n = 6, 8] as potential chelates for ²³⁰U

Stereoselectivity in electron-transfer reactions in chiral media

Synthesis and characterisation of ionic liquids based on 1-butyl-3-methylimidazolium chloride and MCl₄, M = Hf and Zr

First examples of extended structures based on {PMo₁₂Sb₂O₄₀} polyoxoanions

Kinetic and structural studies on ‘tethered’ Ru(ii) arene ketone reduction catalysts

Back cover

Dalton Transactions Vol -0

NMR studies of zinc binding in a multi-histidinic peptide fragment

Content Provider	Royal Society of Chemistry (RSC)
Author	Medici, Serenella Anedda, Roberto Peana, Massimiliano Zoroddu, Maria Antonietta
Copyright Year	2010
Abstract	A multi-histidinic peptide and its minimal models have been investigated for Zn(II) binding. We have used NMR spectroscopy to probe the binding of zinc to the three repeats (T1R2S3R4S5H6T7S8E9G10)3 and to its mono-histidinic minimal models, the 9- and 10-aminoacid fragment. 1H-1H TOCSY, 1H-13C HSQC, 1H-1H NOESY and 1H-1H ROESY multidimensional NMR techniques were performed to understand the details of metal binding sites and the conformational behaviour of the peptides at different pH values and at different ligand to metal molar ratios. Zinc coordination involves imidazole Nδ of His6 and carboxyl γ-O of Glu9 residues; interaction with peptide oxygens of the His6-Thr7 or Thr7-Ser8 bonds in a tetrahedral arrangement with the minimal model peptides, cannot be excluded. Zinc coordination involves, at physiologic pH, all the three imidazole Nδ donors of His6, His16 and His26 as well as carboxyl γ-O of Glu residues in a tetra, penta or octahedral arrangement with the three repeats, the 30-aminoacid fragment. Zinc complexation induces important structural changes with the C-terminal portion of the ligand, constraining it to leave its disordered conformation. Our results give rise to a model of the induced structure of the peptides when bound to zinc. At high pH, amide deprotonation does not take place and hydroxo or high molecular weight polymeric species may be formed.
Starting Page	1282
Ending Page	1294
Page Count	13
File Format	HTM / HTML PDF
ISSN	14779226
Volume Number	39
Issue Number	5
Journal	Dalton Transactions
DOI	10.1039/b914296g
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Glu Peptide bond Nuclear magnetic resonance Zinc Heteronuclear single quantum coherence spectroscopy Roesy (singer) Ligand Concentration Imidazole Glutamic acid C-terminus Protein structure Amide
Content Type	Text
Resource Type	Article
Subject	Inorganic Chemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

ZINC BINDING IN A MULTI-HISTIDINIC PEPTIDE FRAGMENT (2010)

Copper(ii) and zinc(ii) ion binding properties of a MAP type branched ligand with histidines as surface functionalities

STRUCTURAL MODEL OF A Ni(II) COMPLEX WITH A 30-AMINOACID PEPTIDE THROUGH AN NMR STUDY

STRUCTURAL MODEL OF A Ni(II) COMPLEX WITH A 30-AMINOACID PEPTIDE THROUGH AN NMR STUDY

Abstract structural investigations of poly(methyl methacrylate) by two-dimensional nmr (2004).

Bound water in apo and holo bovine heart fatty-acid-binding protein determined by heteronuclear NMR spectroscopy.

Manganese and cobalt binding in a multi-histidinic fragment

381unlocking the structure and flexibility in a four residue peptide using 1h nmr spectroscopy unlocking the structure and flexibility in a four residue peptide using 1h nmr spectroscopy.

Structural differences between the alkali-extracted water-soluble cell wall polysaccharides from mycelial and yeast phases of the pathogenic dimorphic fungus Paracoccidioides brasiliensis

NMR studies of zinc binding in a multi-histidinic peptide fragment

Similar Documents

ZINC BINDING IN A MULTI-HISTIDINIC PEPTIDE FRAGMENT (2010)

Copper(ii) and zinc(ii) ion binding properties of a MAP type branched ligand with histidines as surface functionalities

STRUCTURAL MODEL OF A Ni(II) COMPLEX WITH A 30-AMINOACID PEPTIDE THROUGH AN NMR STUDY

STRUCTURAL MODEL OF A Ni(II) COMPLEX WITH A 30-AMINOACID PEPTIDE THROUGH AN NMR STUDY

Abstract structural investigations of poly(methyl methacrylate) by two-dimensional nmr (2004).

Bound water in apo and holo bovine heart fatty-acid-binding protein determined by heteronuclear NMR spectroscopy.

Manganese and cobalt binding in a multi-histidinic fragment

381unlocking the structure and flexibility in a four residue peptide using 1h nmr spectroscopy unlocking the structure and flexibility in a four residue peptide using 1h nmr spectroscopy.

Structural differences between the alkali-extracted water-soluble cell wall polysaccharides from mycelial and yeast phases of the pathogenic dimorphic fungus Paracoccidioides brasiliensis

NMR studies of zinc binding in a multi-histidinic peptide fragment