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Trimethyl lock: a trigger for molecular release in chemistry, biology, and pharmacology

Artificial chirogenesis: a gateway to new opportunities in material science and catalysis

Exceptional surface area from coordination copolymers derived from two linear linkers of differing lengths

Multielectron redox chemistry of lanthanide Schiff-base complexes

Simultaneous microscale optical manipulation, fabrication and immobilisation in aqueous media

Photochemical tyrosine oxidation with a hydrogen-bonded proton acceptor by bidirectional proton-coupled electron transfer

Comparative analysis of the influence of H-bond strength and solvent on chelate cooperativity in H-bonded supramolecular complexes

Encapsulation of a ‘naked’ Br⁻ anion in a polyoxotitanate host

Bicyclic dinuclear tris-(ditopic diphosphane) complexes of zerovalent group 10 metals

Orthogonal, metal-free surface modification by strain-promoted azide–alkyne and nitrile oxide–alkene/alkyne cycloadditions

The contrasting chemical reactivity of potent isoelectronic iminopyridine and azopyridine osmium(ii) arene anticancer complexes

Accelerated aging: a low energy, solvent-free alternative to solvothermal and mechanochemical synthesis of metal–organic materials

Towards “drug-like” indole-based transmembrane anion transporters

Enantioselective total synthesis of (+)-ibophyllidine via an asymmetric phosphine-catalyzed [3 + 2] annulation

Fe, Co, and Ni ions promote the catalytic activity of amorphous molybdenum sulfide films for hydrogen evolution

Vapour-driven Marangoni propulsion: continuous, prolonged and tunable motion

New tetrathiafulvalene fused-naphthalene diimides for solution-processible and air-stable p-type and ambipolar organic semiconductors

Fluorescent DNAs printed on paper: sensing food spoilage and ripening in the vapor phase

Loading and release capabilities of charged dendronized polymers revealed by EPR spectroscopy

Pair distribution function-derived mechanism of a single-crystal to disordered to single-crystal transformation in a hemilabile metal–organic framework

Prediction on the existence and chemical stability of cuprous fluoride

Design and synthesis of unprecedented cyclic γ-AApeptides for antimicrobial development

Experimental and quantum chemical characterization of the water oxidation cycle catalysed by [Ru^II(damp)(bpy)(H₂O)]²⁺

Two design strategies for enhancement of multilayer–DNA-origami folding: underwinding for specific intercalator rescue and staple-break positioning

3-Vinylborane-functionalized oligothiophenes: isomer-dependent electronic structure and fluorescence enhancement upon anion binding

A new approach for modular polymer–polymer conjugations via Heck coupling

Amplified optical aptasensors through the endonuclease-stimulated regeneration of the analyte

Cavity-induced enantioselectivity reversal in a chiral metal–organic framework Brønsted acid catalyst

Electrochemistry and electrogenerated chemiluminescence of thiophene and fluorene oligomers. Benzoyl peroxide as a coreactant for oligomerization of thiophene dimers

Oxide formation on biological nanostructures via a structure-directing agent: towards an understanding of precise structural transcription

Label-free chemical imaging in vivo: three-dimensional non-invasive microscopic observation of amphioxus notochord through stimulated Raman scattering (SRS)

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Amplified optical aptasensors through the endonuclease-stimulated regeneration of the analyte

Content Provider	Royal Society of Chemistry (RSC)
Author	Lu, Chun-Hua Wang, Fuan Willner, Itamar
Copyright Year	2012
Abstract	A versatile amplified aptamer-based sensing platform is introduced. The method is based on the design of two subunits of the aptamer sequence corresponding to the respective substrate. Each of the subunits is elongated at its 3′ and 5′ ends with complementary sequences that are cleavable in their duplex structures by the endonucleases, PvuII and HaeIII, respectively. One of the subunits is functionalized by a fluorophore, F, and the subunit is blocked through hybridization to a quencher, Q, modified nucleic acid. Blocking of the aptamer subunit prohibits the formation of the aptamer subunit complex, and it leads to the quenching of the fluorophore. In the presence of the target analyte, the blocked aptamer subunit is separated, and the respective aptamer subunits–analyte complex is formed. The endonucleases PvuII and HaeIII cleave the co-stabilizing duplex regions of the aptamer–analyte complex, leading to the separation of the aptamer–substrate complex, the regeneration of the analyte, and the release of the fluorophore. The autonomous release of the fluorophore leads to the amplified optical detection of the analyte. The method is implemented to detect adenosine triphosphate (ATP, detection limit, 20 nM), vasopressin (VP, detection limit, 2 nM), and cocaine (detection limit, 100 nM).
Starting Page	2616
Ending Page	2622
Page Count	7
File Format	HTM / HTML PDF
ISSN	20416520
Volume Number	3
Issue Number	8
Journal	Chemical Science
DOI	10.1039/c2sc20426f
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Analyte Acid Fluorophore Aptamer Cocaine ATP Adenosine triphosphate Vasopressin
Content Type	Text
Resource Type	Article
Subject	Chemistry

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Optical Aptasensors for Adenosine Triphosphate.

Exact tailoring of an ATP controlled streptavidin binding aptamer

Novel autonomous protein-encoded aptamer nanomachines and isothermal exponential amplification for ultrasensitive fluorescence polarization sensing of small molecules

Principal factors that determine the extension of detection range in molecular beacon aptamer/conjugated polyelectrolyte bioassays

pH- and ligand-induced release of loads from DNA–acrylamide hydrogel microcapsules

Graphene oxide as a nano-platform for ATP detection based on aptamer chemistry

Optimization of Aptamer-Based Electrochemical Biosensor for ATP Detection Using Screen-Printed Carbon Electrode/Gold Nanoparticles (SPCE/AuNP)

Reversible bioresponsive aptamer-based nanocomposites: ATP binding and removal from DNA-grafted silica nanoparticles

Programmed dissociation of dimer and trimer origami structures by aptamer–ligand complexes

Amplified optical aptasensors through the endonuclease-stimulated regeneration of the analyte

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