NDLI: Evaluation of intracellular telomerase activity through cascade DNA logic gates

Content Provider	Royal Society of Chemistry (RSC)
Author	Huang, Shan Bi, Sai Wang, Wenjing Zhang, Jian-Rong Rui, Kai Zhu, Jun-Jie Li, Jingjing
Copyright Year	2017
Abstract	Telomerase plays a vital role in cancer and aging, and telomerase activity detection has drawn great attention recently. However, a feasible in situ imaging system for intracellular telomerase is still a challenge. Here, we develop a novel approach to image intracellular telomerase activity using DNA-based computation. A cascade nucleic acid logic gate that responded to intracellular telomerase was constructed. A telomerase substrate (TS) probe, extended by intracellular telomerase, worked as an input to initiate computation cascades. In this way, intracellular telomerase could be clearly indicated by fluorophore labeled nucleic acids as the output. Through one-step incubation, evaluation of the intracellular telomerase activity for a HeLa cell line and the ability to differentiate cancer cells from normal cells could be realized. Furthermore, the response of intracellular telomerase activity to a telomerase-inhibiting model drug was observed using the proposed method. Thus, this intracellular telomerase computation device will allow improvements in studying the relationship between telomerase and cancer, and may help to develop telomerase inhibitors. This finding also expands the applications of DNA computational techniques in cells.
Starting Page	174
Ending Page	180
Page Count	7
File Format	HTM / HTML PDF
ISSN	20416520
Volume Number	8
Issue Number	1
Journal	Chemical Science
DOI	10.1039/c6sc01953f
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	HeLa Acid Fluorophore DNA Logic gate Telomerase Cancer
Content Type	Text
Resource Type	Article
Subject	Chemistry

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