NDLI: <sup>19</sup>F MRI detection of β-galactosidase activity for imaging of gene expression

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Engineering DNA aptamers for novel analytical and biomedical applications

Novel and efficient templates for assembly of rotaxanes and catenanes

High-contrast Cu(i)-selective fluorescent probes based on synergistic electronic and conformational switching

Does supramolecular ordering influence exciton transport in conjugated systems? Insight from atomistic simulations

A chemically-controlled supramolecular protein polymer formed by a myoglobin-based self-assembly system

A mononuclear nonheme iron(iv)-oxo complex which is more reactive than cytochrome P450 model compound I

A redox-active reverse donor–acceptor bistable [2]rotaxane

Cation vacancy order in the K_0.8+xFe_1.6−ySe₂ system: Five-fold cell expansion accommodates 20% tetrahedral vacancies

Immobilised photosensitisers for continuous flow reactions of singlet oxygen in supercritical carbon dioxide

Thermotropic liquid crystals based on 1,8,9,16-tetrasubstituted tetraphenylenes and their structure–property relationship studies

Catalytic, enantioselective synthesis of stilbene cis-diamines: A concise preparation of (−)-Nutlin-3, a potent p53/MDM2 inhibitor

All-optical fluorescence image recovery using modulated stimulated emission depletion

Benzoquinone-derived sulfinyl imines as versatile intermediates for alkaloid synthesis: Total synthesis of (–)-3-demethoxyerythratidinone

Kinetics-driven high power Li-ion battery with a-Si/NiSi_x core-shell nanowire anodes

Isotope effect on ultrafast charge-transfer-to-solvent reaction from I⁻ to water in aqueous NaI solution

Cell-compatible, integrin-targeted cryptophane-¹²⁹Xe NMR biosensors

Energy versuselectron transfer in organic solar cells: a comparison of the photophysics of two indenofluorene: fullerene blend films

A hybrid magnet with coexistence of ferromagnetism and photoinduced Fe(iii) spin-crossover

Heavy water hydration of mannose: the anomeric effect in solvation, laid bare

Highly enantioselective palladium-catalyzed umpolung allylation of aldehydes

Spiro-2,2′-bichroman-based bisoxazoline (SPANbox) ligands for Zn^II-catalyzed enantioselective hydroxylation of β-keto esters and 1,3-diester

Effect of anchoring groups in zinc phthalocyanine on the dye-sensitized solar cell performance and stability

¹⁹F MRI detection of β-galactosidase activity for imaging of gene expression

A dendrimer-based platform for simultaneous dual fluorescence imaging of hydrogen peroxide and pH gradients produced in living cells

Molecular coolers: The case for [Cu^II₅Gd^III₄]

The chemical vapor deposition of Cu₂ZnSnS₄ thin films

Chemical tuning of CO₂ sorption in robust nanoporous organic polymers

Orthogonal processing: A new strategy for organic electronics

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¹⁹F MRI detection of β-galactosidase activity for imaging of gene expression

Content Provider	Royal Society of Chemistry (RSC)
Author	Matsushita, Hisashi Shirakawa, Masahiro Mizukami, Shin Sugihara, Fuminori Kikuchi, Kazuya Takikawa, Rika
Copyright Year	2011
Abstract	Imaging of gene expression by magnetic resonance imaging (MRI) yields direct information regarding living systems that cannot be obtained via other methods. In this study, we report the rational design and synthesis of a novel 19F MRI probe that detects β-galactosidase (β-gal) activity, enabling the imaging of gene expression in cells. The 19F MRI signal of the probe was quenched by the intramolecular paramagnetic resonance enhancement from a Gd3+ ion. A contrivance was made in the probe structure to recover the 19F MRI signal after hydrolysis by β-gal with a following self-immolative reaction. This 19F MRI signal change was observed in the physiological aqueous condition. The probe could also detect β-gal activity in fixed HEK293T cells. In conclusion, this new probe enables the 19F MRI detection of cellular gene expression. The probe design strategy is also expected to lead to the development of MRI probes for a wide variety of hydrolase activities.
Starting Page	1151
Ending Page	1155
Page Count	5
File Format	HTM / HTML PDF
ISSN	20416520
Volume Number	2
Issue Number	6
Journal	Chemical Science
DOI	10.1039/c1sc00071c
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Hydrolysis Isotopes of fluorine Beta-galactosidase Gene Magnetic resonance imaging Hydrolase Paramagnetism Ion Nuclear magnetic resonance HEK 293 cells
Content Type	Text
Resource Type	Article
Subject	Chemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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Fluorine polymer probes for magnetic resonance imaging: quo vadis?

Nuclear magnetic resonance decoupling by paramagnetic ion co-ordination at oxygen

19F-MRI for monitoring human NK cellsin vivo

¹⁹F-MRS/¹H-MRI dual-function probe for detection of β-galactosidase activity

Spin-crossover and high-spin iron(ii) complexes as chemical shift ¹⁹F magnetic resonance thermometers

Amyloid imaging using fluorine-19 magnetic resonance imaging (19F-MRI)

In Situ Generated Novel 1H MRI Reporter for β-Galactosidase Activity Detection and Visualization in Living Tumor Cells

Multifunctional hyperbranched polymers for CT/¹⁹F MRI bimodal molecular imaging

A bisphosphonate for 19F-magnetic resonance imaging

¹⁹F MRI detection of β-galactosidase activity for imaging of gene expression

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