NDLI: Silk fibroin gelation <i>via</i> non-solvent induced phase separation

Please wait, while we are loading the content...

Tri-membrane nanoparticles produced by combining liposome fusion and a novel patchwork of bicelles to overcome endosomal and nuclear membrane barriers to cargo delivery

Curcumin loaded mesoporous silica: an effective drug delivery system for cancer treatment

Silk fibroin gelation via non-solvent induced phase separation

LAPONITE®-stabilized iron oxide nanoparticles for in vivo MR imaging of tumors

Chemically modified inulin microparticles serving dual function as a protein antigen delivery vehicle and immunostimulatory adjuvant

Systemic and tumor-targeted delivery of siRNA by cyclic NGR and isoDGR motif-containing peptides

Oseltamivir-conjugated polymeric micelles prepared by RAFT living radical polymerization as a new active tumor targeting drug delivery platform

Multifunctional oligomer incorporation: a potent strategy to enhance the transfection activity of poly(l-lysine)

Back cover

Biomaterials Science Vol - 4 Issue - 2

Biomaterials Science Vol -3

Biomaterials Science Vol -2

Biomaterials Science Vol -1

Silk fibroin gelation via non-solvent induced phase separation

Content Provider	Royal Society of Chemistry (RSC)
Author	Hawkins, Nicholas Kubies, Dana Kasoju, Naresh Pop-Georgievski, Ognen Vollrath, Fritz
Copyright Year	2016
Abstract	Tissue engineering benefits from novel materials with precisely tunable physical, chemical and mechanical properties over a broad range. Here we report a practical approach to prepare Bombyx mori silk fibroin hydrogels using the principle of non-solvent induced phase separation (NIPS). A combination of reconstituted silk fibroin (RSF) and methanol (non-solvent), with a final concentration of 2.5% w/v and 12.5% v/v respectively, maintained at 22 °C temperature turned into a hydrogel within 10 hours. Freeze-drying of this gel gave a foam with a porosity of 88%, a water uptake capacity of 89% and a swelling index of 8.6. The gelation kinetics and the loss tangent of the gels were investigated by rheometry. The changes in the morphology of the porous foams were visualized by SEM. The changes in RSF chemical composition and the relative fraction of its secondary structural elements were analyzed by ATR-FTIR along with Fourier self-deconvolution. And, the changes in the glass transition temperature, specific heat capacity and the relative fraction of crystallinity of RSF were determined by TM-DSC. Data suggested that RSF–water–methanol behaved as a polymer–solvent–non-solvent ternary phase system, wherein the demixing of the water–methanol phases altered the thermodynamic equilibrium of RSF–water phases and resulted in the desolvation and eventual separation of the RSF phase. Systematic analysis revealed that both gelation time and the properties of hydrogels and porous foams could be controlled by the ratios of RSF and non-solvent concentration as well as by the type of non-solvent and incubation temperature. Due to the unique properties we envisage that the herein prepared NIPS induced RSF hydrogels and porous foams can possibly be used for the encapsulation of cells and/or for the controlled release of both hydrophilic and hydrophobic drugs.
Starting Page	460
Ending Page	473
Page Count	14
File Format	HTM / HTML PDF
ISSN	20474830
Volume Number	4
Issue Number	3
Journal	Biomaterials Science
DOI	10.1039/c5bm00471c
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Dielectric loss RSF Tissue engineering Heat capacity Bombyx mori Hydrophobe Thermodynamic equilibrium Gel Glass transition Fourier FTIR Fibroin Porosity Hydrophile Freeze-drying Methanol
Content Type	Text
Resource Type	Article
Subject	Materials Science Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A silk fibroin hydrogel with reversible sol–gel transition

Preparation of porous scaffolds from silk fibroin extracted from the silk gland of bombyx mori (b. Mori) (2012).

Effect of solvent concentration on morphology of electrospun Bombyx mori silk

Gelation behavior of Antheraea pernyi silk fibroin

Silk Fibroin Based Porous Materials

Bombyx mori Silk Fibroin Regeneration in Solution of Lanthanide Ions: A Systematic Investigation

The effect of water on the conformation transition of Bombyx mori silk fibroin

Study on porous silk fibroin materials. I. Fine structure of freeze dried silk fibroin

The extraction of fibroin protein from Bombyx mori silk cocoon: Optimization of process parameters

Silk fibroin gelation via non-solvent induced phase separation

Similar Documents

A silk fibroin hydrogel with reversible sol–gel transition

Preparation of porous scaffolds from silk fibroin extracted from the silk gland of bombyx mori (b. Mori) (2012).

Effect of solvent concentration on morphology of electrospun Bombyx mori silk

Gelation behavior of Antheraea pernyi silk fibroin

Silk Fibroin Based Porous Materials

Bombyx mori Silk Fibroin Regeneration in Solution of Lanthanide Ions: A Systematic Investigation

The effect of water on the conformation transition of Bombyx mori silk fibroin

Study on porous silk fibroin materials. I. Fine structure of freeze dried silk fibroin

The extraction of fibroin protein from Bombyx mori silk cocoon: Optimization of process parameters

Silk fibroin gelation via non-solvent induced phase separation