NDLI: Molecular farming of fluorescent virus-based nanoparticles for optical imaging in plants, human cells and mouse models

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Molecular farming of fluorescent virus-based nanoparticles for optical imaging in plants, human cells and mouse models

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Biomaterials Science Vol - 2 Issue - 4

Biomaterials Science Vol - 2 Issue - 3

Biomaterials Science Vol - 2 Issue - 2

Biomaterials Science Vol -1

Molecular farming of fluorescent virus-based nanoparticles for optical imaging in plants, human cells and mouse models

Content Provider	Royal Society of Chemistry (RSC)
Author	Commandeur, U. Fischer, R. Dickmeis, C. Nagarajan, A. S. Shukla, S. Steinmetz, N. F.
Copyright Year	2014
Abstract	The application of plant virus-derived nanostructures in materials science, biomedical research and engineering has recently been promoted by the development of fluorescence-labeled viruses for optical imaging in tissue culture and preclinical animal models. Most studies reported thus far have focused on the application of viruses that have been chemically modified with organic dyes. In this investigation, we sought to develop and study genetically-engineered virus-based biomaterials that incorporate green or red fluorescent proteins. The genetic introduction of such imaging moieties is advantageous because post-harvest modifications are not required, thus minimizing the number of manufacturing steps and maximizing the yields of each fluorescent probe. Specifically, we engineered the filamentous plant virus Potato virus X (PVX) to display green fluorescent protein (GFP) or mCherry as N-terminal coat protein (CP) fusions, producing a 1 : 3 fusion protein to CP ratio. The infection of Nicotiana benthamiana plants with the recombinant GFP-PVX and mCherry-PVX particles was documented by fluorescence imaging, structural analysis and genetic characterization to determine the stability of the chimeras and optimize the molecular farming protocols. We also demonstrated the application of fluorescent mCherry-PVX filaments as probes for optical imaging in human cancer cells and a preclinical mouse model. Cell viability assays and histological analysis following the administration of mCherry-PVX indicated the biocompatibility and rapid tissue clearance of the particles. Such particles could therefore be functionalized with additional cancer-specific detection ligands to provide tools for molecular imaging, allowing the investigation of molecular signatures, disease progression/recurrence and the efficacy of novel therapies.
Starting Page	784
Ending Page	797
Page Count	14
File Format	HTM / HTML PDF
ISSN	20474830
Volume Number	2
Issue Number	5
Journal	Biomaterials Science
DOI	10.1039/c3bm60277j
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Nicotiana benthamiana Medical optical imaging Genetically modified mouse Tissue culture Fluorescence Materials science Plant virus Organic farming Protein N-terminus The Infection Potato virus X Green fluorescent protein Biocompatibility Biomaterial MCherry Fusion protein Cancer
Content Type	Text
Resource Type	Article
Subject	Materials Science Biomedical Engineering

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Long-Term Potato Virus X (PVX)-Based Transient Expression of Recombinant GFP Protein in Nicotiana benthamiana Culture In Vitro

Constraints to virus infection in Nicotiana benthamiana plants transformed with a potyvirus amplicon

ORF6 of Tobacco mosaic virus is a determinant of viral pathogenicity in Nicotiana benthamiana (2004)

High Levels of Expression of Fibroblast Growth Factor 21 in Transgenic Tobacco (Nicotiana benthamiana)

Inverse correlation between mRNA levels in GFP-silenced transgenic Nicotiana benthamiana and resistance to Potato virus X engineered to contain GFP sequence

Pre-Symptomatic Detection of Viral Infection in Tobacco Leaves Using PAM Fluorometry

Development of Recombinant Protein-Based Vaccine Against Classical Swine Fever Virus in Pigs Using Transgenic Nicotiana benthamiana

Use of a vector based on potato virus x in a whole plant assay to demonstrate nuclear targeting of potato spindle tuber viroid.

Systemic Infection of Nicotiana benthamiana with Potato virus X Nanoparticles Presenting a Fluorescent iLOV Polypeptide Fused Directly to the Coat Protein

Molecular farming of fluorescent virus-based nanoparticles for optical imaging in plants, human cells and mouse models

Similar Documents

Long-Term Potato Virus X (PVX)-Based Transient Expression of Recombinant GFP Protein in Nicotiana benthamiana Culture In Vitro

Constraints to virus infection in Nicotiana benthamiana plants transformed with a potyvirus amplicon

ORF6 of Tobacco mosaic virus is a determinant of viral pathogenicity in Nicotiana benthamiana (2004)

High Levels of Expression of Fibroblast Growth Factor 21 in Transgenic Tobacco (Nicotiana benthamiana)

Inverse correlation between mRNA levels in GFP-silenced transgenic Nicotiana benthamiana and resistance to Potato virus X engineered to contain GFP sequence

Pre-Symptomatic Detection of Viral Infection in Tobacco Leaves Using PAM Fluorometry

Development of Recombinant Protein-Based Vaccine Against Classical Swine Fever Virus in Pigs Using Transgenic Nicotiana benthamiana

Use of a vector based on potato virus x in a whole plant assay to demonstrate nuclear targeting of potato spindle tuber viroid.

Systemic Infection of Nicotiana benthamiana with Potato virus X Nanoparticles Presenting a Fluorescent iLOV Polypeptide Fused Directly to the Coat Protein

Molecular farming of fluorescent virus-based nanoparticles for optical imaging in plants, human cells and mouse models