NDLI: Scaffold free fabrication of linear multicellular assemblies by dielectrophoretic hydrogel trapping technique

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Scaffold free fabrication of linear multicellular assemblies by dielectrophoretic hydrogel trapping technique

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Biomaterials Science Vol - 1 Issue - 8

Biomaterials Science Vol - 1 Issue - 7

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Scaffold free fabrication of linear multicellular assemblies by dielectrophoretic hydrogel trapping technique

Content Provider	Royal Society of Chemistry (RSC)
Author	Small, William R. Stoyanov, Simeon D. Paunov, Vesselin N.
Copyright Year	2013
Abstract	We have designed a scaffold-free cell assembly method which can produce linear structures of individual living cells without using templates. The method involves dielectrophoretic assembly of cells suspended in a solution of a gelling agent above the gelling temperature. After the cell assembly in string-like structures was achieved with the AC electric field still applied, we gelled the solution by cooling it below its gelling point. The hydrogel entraps the assembled cell structure, preventing its disassembly and allowing further analysis without the presence of the external electric field. We pre-functionalised the cells with polyelectrolytes before the dielectrophoretic assembly which allowed us to line them up in multicellular strings and bind them together with oppositely charged polyelectrolyte after the gel formation. Finally, by dissolving the hydrogel, we released the linear chains of living cells which were collected and studied by optical and fluorescence microscopy. Cell viability tests with fluorescein diacetate confirmed that the cells in the formed worm-like structures remain viable after the cell assembly procedure. The linear cell aggregates are stable without the electric field and can be further cultured or treated with additional polyelectrolytes which make the method attractive for tissue engineering. We envisage that this technique could find possible applications for assembly of neuron cells in linear structures and more complex cell networks.
Starting Page	996
Ending Page	1002
Page Count	7
File Format	HTM / HTML PDF
ISSN	20474830
Volume Number	1
Issue Number	9
Journal	Biomaterials Science
DOI	10.1039/c3bm60118h
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	String theory Polyelectrolyte AC Thickening agent Electric field Neuron Tissue engineering Fluorescein Fluorescence microscope Complex cell Acetate Gel
Content Type	Text
Resource Type	Article
Subject	Materials Science Biomedical Engineering

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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