NDLI: Separation of lipopolysaccharides containing different fatty acid chains using hydrophobic interaction chromatography

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Separation of lipopolysaccharides containing different fatty acid chains using hydrophobic interaction chromatography

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Back cover

Analytical Methods Vol - 4 Issue - 2

Analytical Methods Vol -3

Analytical Methods Vol -2

Analytical Methods Vol -1

Separation of lipopolysaccharides containing different fatty acid chains using hydrophobic interaction chromatography

Content Provider	Royal Society of Chemistry (RSC)
Author	Li, Ye Ren, Ke Shi, Feng Wang, Xiaoyuan
Copyright Year	2012
Abstract	Lipopolysaccharides, known as endotoxins, are major components in the outer membrane of most Gram-negative bacteria. Because of their amphipathic property, it is difficult to separate lipopolysaccharides. In this study, hydrophobic interaction chromatography was used for the separation of lipopolysaccharides containing different numbers of fatty acid chains. Three species of lipopolysaccharides were extracted from Escherichia coli W3110, MLK1067, and MKV15b, respectively. Their interactions with three types of hydrophobic matrixes Butyl-Sepharose, Phenyl-Sepharose and Octyl-Sepharose were investigated. Several organic solvents were used to elute the bound lipopolysaccharides from different matrixes. Butyl-Sepharose, propyl alcohol and 0.8 mol l−1 sodium acetate (pH 4.5) loading buffer were chosen for the best separation system, and lipopolysaccharides containing different fatty acid chains were separated. This system might be useful for removing the contaminated lipopolysaccharides from proteins produced by E. coli, or separating different species of lipopolysaccharides.
Starting Page	838
Ending Page	843
Page Count	6
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	4
Issue Number	3
Journal	Analytical Methods
DOI	10.1039/c2ay05663a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Fatty acid Escherichia coli PH Amphiphile Gram-negative bacteria Hydrophobe Chromatography Sodium acetate
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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