NDLI: Mass spectrometric imaging as a high-spatial resolution tool for functional genomics: Tissue-specific gene expression of <i>TT7</i> inferred from heterogeneous distribution of metabolites in <i>Arabidopsis</i> flowers

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Mass spectrometric imaging as a high-spatial resolution tool for functional genomics: Tissue-specific gene expression of TT7 inferred from heterogeneous distribution of metabolites in Arabidopsis flowers

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Analytical Methods Vol -3

Analytical Methods Vol -2

Analytical Methods Vol -1

Mass spectrometric imaging as a high-spatial resolution tool for functional genomics: Tissue-specific gene expression of TT7 inferred from heterogeneous distribution of metabolites in Arabidopsis flowers

Content Provider	Royal Society of Chemistry (RSC)
Author	Lee, Young Jin Nikolau, Basil J. Korte, Andrew R. Song, Zhihong
Copyright Year	2012
Abstract	Laser desorption/ionization (LDI) mass spectrometry imaging (MSI) was used to acquire chemical images of flavonoid metabolites on the surface of wild-type and mutant (tt7) Arabidopsis thaliana flowers. Flavonoids were localized to the petals and carpels of flowers, with tissue heterogeneity in the petals. Specifically, kaempferol and/or its glycosides were abundant in the distal region of petals and quercetin and its downstream flavonoids were highly enriched in the more proximal region of petals. As a result of a mutation in the TT7 gene which blocks the conversion of dihydrokaempferol to dihydroquercetin, the downstream metabolites, quercetin, isohamnetin, and their glycosides, were not observed in the mutant flowers. Instead, the metabolites in an alternative pathway, kaempferol and/or its glycosides, were as highly abundant on the proximal region of the petals as in the distal region. In addition, the combined flavonoid amounts on the proximal region of petals in the wild-type are almost equivalent to the amounts of kaempferol and/or its glycosides in the mutant. This strongly suggests that the expression of the TT7 gene is localized on the proximal part of the petal while the other genes in the upper stream pathway are evenly expressed throughout the petal. Most importantly, this work demonstrates MSI of metabolites can be utilized for the localization of gene expression.
Starting Page	474
Ending Page	481
Page Count	8
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	4
Issue Number	2
Journal	Analytical Methods
DOI	10.1039/c2ay05618f
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	MSI Kaempferol Mass spectrometry imaging Aromadendrin Flavonoid LDI Arabidopsis thaliana Gene Ionization TT7 Quercetin Mutation Petal
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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