NDLI: White light excitation fluorescence (WLEF)Part I. Exploring the use in analytical fluorimetry

Content Provider	Royal Society of Chemistry (RSC)
Author	Mishra, Ashok Kumar Prakash, John
Copyright Year	2011
Abstract	The hitherto unexplored scope of white light excitation fluorescence (WLEF) in analytical fluorimetry has been examined. In WLEF, the entire UV-Visible spectrum is used as the excitation source instead of selecting a particular wavelength/band pass excitation (BPE) source as is commonly done in conventional fluorescence spectroscopic methods. It is found that WLEF gives good spectral integrity, analytical sensitivity, intensity enhancement in the analysis of transparent fluorescence solutions and in the highly scattering medium with the use of difference spectrum. WLEF is shown to be a two-dimensional representation of the three-dimensional excitation–emission–matrix fluorescence (EEMF) spectrum. One of the major advantages of WLEF is that there is no need to know the excitation wavelength of an analyte, and the technique is cost saving as there is no need for band pass/cut off filters and/or a monochromator.
Starting Page	362
Ending Page	367
Page Count	6
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	3
Issue Number	2
Journal	Analytical Methods
DOI	10.1039/c0ay00703j
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Fluorescence spectroscopy Analyte Monochromator Fluorescence WLEF Wavelength
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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White light excitation fluorescence (WLEF)Part I. Exploring the use in analytical fluorimetry