NDLI: Novel method for the extraction and identification of microplastics in ocean trawl and fish gut matrices

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Analytical Methods Vol -9

Analytical Methods Vol - 9 Issue - 18

Analytical Methods Vol - 9 Issue - 9

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On the harmonization of methods for measuring the occurrence, fate and effects of microplastics

Analytical challenges associated with the determination of microplastics in the environment

Improving microplastics source apportionment: a role for microplastic morphology and taxonomy?

Some problems and practicalities in design and interpretation of samples of microplastic waste

Sampling, isolating and identifying microplastics ingested by fish and invertebrates

Methods of analysing chemicals associated with microplastics: a review

A review of analytical techniques for quantifying microplastics in sediments

Identification methods in microplastic analysis: a review

Incorporating citizen science to study plastics in the environment

The human dimension: how social and behavioural research methods can help address microplastics in the environment

Extraction and identification of microplastic particles from secondary wastewater treatment plant (WWTP) effluent

A novel, density-independent and FTIR-compatible approach for the rapid extraction of microplastics from aquatic sediments

A risk framework for tackling marine debris

Optimisation of enzymatic digestion and validation of specimen preservation methods for the analysis of ingested microplastics

Grab vs. neuston tow net: a microplastic sampling performance comparison and possible advances in the field

Quantifying ingested debris in marine megafauna: a review and recommendations for standardization

An approach for extraction, characterization and quantitation of microplastic in natural marine snow using Raman microscopy

Novel method for the extraction and identification of microplastics in ocean trawl and fish gut matrices

Validation of density separation for the rapid recovery of microplastics from sediment

An automated approach for microplastics analysis using focal plane array (FPA) FTIR microscopy and image analysis

First detection of seven phthalate esters (PAEs) as plastic tracers in superficial neustonic/planktonic samples and cetacean blubber

Extracting DNA from ocean microplastics: a method comparison study

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Novel method for the extraction and identification of microplastics in ocean trawl and fish gut matrices

Content Provider	Royal Society of Chemistry (RSC)
Author	Ghosal, Sutapa Wall, Stephen Wang, Zhong-Min Gassel, Margy Wagner, Jeff Rochman, Chelsea
Copyright Year	2017
Abstract	This work presents alternative extraction and analysis techniques to identify microplastics in the environment. This study aims to address previously noted issues with methods that use aggressive extraction treatments or optical microscopy identification techniques alone. Pulsed ultrasonic extraction with ultrapure water was used to remove microplastics from fish stomachs without dissolving the stomach tissues or microplastics. The technique is relatively simple and minimizes issues with hazardous disposal and laboratory safety. Microplastics were characterized using optical microscopy, scanning electron microscopy plus energy-dispersive X-ray spectroscopy (SEM/EDS), Fourier transform infrared (FTIR) micro-spectroscopy, and Raman micro-spectroscopy (RMS). These methods were demonstrated successfully on laboratory fish exposed to reference microplastics and on ocean surface trawl and fish samples taken from subtropical gyres. Polyethylene (PE), polypropylene (PP), and blended PE + PP microplastics were detected in the stomachs of ocean-caught lanternfish, with the majority consisting of PE. One nearly empty lanternfish stomach contained a long PE fiber that appeared to block the digestive tract. Minor amounts of fat, proteins, and carbohydrates were detected by FTIR on many microplastic surfaces. The Pacific Ocean trawl samples yielded similar plastic compositions as the fish stomachs, plus one polystyrene particle. Of the 115 ocean particles analyzed by FTIR (15 μm to 5 mm), 25 particles were microplastics (600 μm to 5 mm). The microplastic PE + PP copolymer blends were the most visibly degraded of the four observed types. FTIR and SEM/EDS identified micro-shell pieces in the ocean fish stomachs that resembled microplastics by optical microscopy alone.
Starting Page	1479
Ending Page	1490
Page Count	12
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	9
Issue Number	9
Journal	Analytical Methods
DOI	10.1039/c6ay02396g
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Spectroscopy Infrared spectroscopy Polyethylene Energy-dispersive X-ray spectroscopy Trawling Optical microscope Fourier transform Polystyrene Scanning electron microscope Root mean square Lipid Laboratory safety Fourier Polypropylene FTIR Micrometre Raman Pacific Ocean Microplastics Lanternfish Copolymer Ultrapure water The Technique Ultrasound
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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Novel method for the extraction and identification of microplastics in ocean trawl and fish gut matrices

Abundance and distribution of microplastics in tropical estuarine mangrove areas around Penang, Malaysia

Infrared thermochromic properties of monoclinic VO₂ nanopowders using a malic acid-assisted hydrothermal method for adaptive camouflage

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Novel method for the extraction and identification of microplastics in ocean trawl and fish gut matrices

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