NDLI: The preparation of an electrochemiluminescence (ECL) active DNA nanoprobe for label-free and amplified ECL sensing of microRNA

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The preparation of an electrochemiluminescence (ECL) active DNA nanoprobe for label-free and amplified ECL sensing of microRNA

Content Provider	Royal Society of Chemistry (RSC)
Author	Guo, Zhihui Zheng, Xingwang Yao, Xiunan
Copyright Year	2017
Abstract	In this work, we report a new scheme to prepare a DNA nanoprobe by adsorbing oligonucleotides on the surface of electrochemiluminescence (ECL) active chitosan/Ru(bpy)32+/silica nanoparticles (CRuS NPs). Then, the characteristics of the DNA nanoprobes were also investigated by transmission electron microscopy (TEM), gel electrophoresis (GE), and zeta potential and contact angle experiments. Our results showed that, compared to the preparation schemes of previously reported nanoprobes, this new scheme is simple and general. More importantly, the proposed DNA nanoprobes also presented the features of rapidity, specificity and sensitivity for binding to target miRNA. In the initial state of the DNA nanoprobes, the DNA nanoprobes were hardly adsorbed on a Nafion/MWNT modified electrode, and only a very low ECL signal was detected from the ECL active CRuS NPs. However, while target miRNA was present in DNA nanoprobe solution, target miRNA could hybridize with DNA probes on the DNA nanoprobe surface. As a result, this reaction forced the DNA probes away from the surface of the nanoprobes. Rigid structured double stranded DNA-miRNA products and naked CRuS NPs were formed in the solution. In this case, based on the strong affinity interaction of CRuS NPs with the Nafion film, these naked CRuS NPs could effectively be pre-concentrated on the Nafion/MWNT modified electrode and produce a stronger ECL signal. Based on this ECL signal difference, this new ECL DNA nanoprobe could detect miRNA at a 0.03 pM level and was also successfully used for miRNA detection in serum samples.
Starting Page	312
Ending Page	321
Page Count	10
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	9
Issue Number	2
Journal	Analytical Methods
DOI	10.1039/c6ay02807a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Carbon nanotube Transmission electron microscopy Electrode Gel electrophoresis Zeta potential Nafion DNA Electrochemiluminescence Chitosan Contact angle Silicon dioxide ECL
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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The preparation of an electrochemiluminescence (ECL) active DNA nanoprobe for label-free and amplified ECL sensing of microRNA

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