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Analytical Methods Vol - 2 Issue - 7

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Analytical Methods Vol -1

Impedimetric approach for monitoring bacterial cultures based on the changes in the magnitude of the interface capacitance

Content Provider	Royal Society of Chemistry (RSC)
Author	Muñoz-Berbel, Xavier García-Aljaro, Cristina Cortina-Puig, Montserrat Muñoz, Francesc Xavier Vigués, Núria Escudé, Roger Mas, Jordi
Copyright Year	2010
Abstract	A previously reported methodology is applied to monitor the concentration of bacterial suspensions from an incubator using impedance spectroscopy and platinum electrodes. The interface capacitance, commonly fitted as a constant phase element, CPEi, was found sensitive to the suspended bacteria concentration after short exposure times in a wide range of concentrations (from 102 to 107 colony forming units per mL, CFU mL−1) with a limit of detection of 10 CFU mL−1. The effect of the substances released during bacterial growth (enzymes, toxins, exopolysaccharide, etc.) in the CPEi magnitude was found to be negligible and samples only containing cells washed with saline solutions showed results comparable to those obtained using aliquots directly extracted from the bacterial incubator. This impedimetric approach showed correlation with classical microbiological methods for measuring bacterial concentration such as plating onto agar, optical density and fluorescence microscopy. The impedimetric approach was simplified to a single frequency analysis by selecting the optimal frequency for the measurement.
Starting Page	1036
Ending Page	1042
Page Count	7
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	2
Issue Number	8
Journal	Analytical Methods
DOI	10.1039/c0ay00050g
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Spectroscopy Extracellular polymeric substance Constant phase element Ant colony Agar Fluorescence microscope Absorbance CFU Classical mechanics Frequency analysis Capacitance Bacteria Electrical impedance
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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