NDLI: Improving the depth of coverage in membrane proteomic studies through the use of lipid-based protein immobilization technology in parallel with methanol-facilitated solubilisation

Please wait, while we are loading the content...

Rapid determination of six kavalactones in kava root and rhizome samples using Fourier transform infrared spectroscopy and multivariate analysis in comparison with gas chromatography

Amperometric detection at carbon felt electrodes. Application to the determination of nitro musk derivatives and phenolic endocrine disruptors

Flow injection analysis of paracetamol using a biomimetic sensor as a sensitive and selective amperometric detector

Optimization and validation of spectrofluorimetric method for the determination of clomipramine hydrochloride via ion-pair complexation with alizarin red S

Sensitive surfactant-mediated spectrofluorimetric determination of sildenafil

HPTLC method development and validation: Quantification of paliperidone in formulations and in vitro release study

Chromatographic analysis of major non-structural carbohydrates in several wood species – an analytical approach for higher accuracy of data

Improving the depth of coverage in membrane proteomic studies through the use of lipid-based protein immobilization technology in parallel with methanol-facilitated solubilisation

A simple strategy for preparation of spherical silica-supported porous chitosan matrix based on sol–gel reaction and simple treatment with ammonia solution

Molecularly imprinted polymer stir bar sorption extraction and electrospray ionization tandem mass spectrometry for determination of 2-aminothiazoline-4-carboxylic acid as a marker for cyanide exposure in forensic urine analysis

Determination of trace quantity of aluminium in dialysate concentrates using solid phase and cloud point extraction methods

Determination of hippuric acid in biological fluids using single drop liquid–liquid-liquid microextraction

Spontaneous water cleanup using an epoxy-based polymer monolith

A practical method for determination of molybdenite Re-Os age by inductively coupled plasma-mass spectrometry combined with Carius tube-HNO₃ digestion

Back matter

Back cover

Analytical Methods Vol - 2 Issue - 4

Analytical Methods Vol - 2 Issue - 3

Analytical Methods Vol - 2 Issue - 2

Analytical Methods Vol -1

Improving the depth of coverage in membrane proteomic studies through the use of lipid-based protein immobilization technology in parallel with methanol-facilitated solubilisation

Content Provider	Royal Society of Chemistry (RSC)
Author	Hofgärtner, Wolfgang T. Hariri, Robert J. Padliya, Neerav D. Bhatia, Mohit B.
Copyright Year	2010
Abstract	Lipid-based protein immobilization (LPI) technology is a platform recently developed to facilitate shotgun membrane proteomic studies based on a nanotechnology framework. Proteoliposomes are generated from a membraneprotein preparation. These proteoliposomes are immobilized onto an LPI chip and then subjected to proteolysis. The proteolytic peptides are then subjected to LC/MS analysis after fractionation by SCX chromatography. The focus of this study was to evaluate how the depth of coverage of the membrane proteome of a particular cell type varied as a function of the sample preparation method used. Human dermal fibroblasts (hDFs) and human bone marrow mesenchymal stem cells (BM-hMSCs) were subjected to membrane proteomic studies using two different sample preparation methods: LPI technology and methanol-facilitated solubilisation. The number of membraneproteins that could be identified from hDFs and BM-hMSCs using LC/MS was greater using LPI technology than it was using methanol-facilitated solubilisation. However, the number of membraneprotein identifications that could be made for both hDFs and BM-hMSCs increased by ∼50% when both sample preparation methods were used in parallel and the MS/MS data was convolved together. Therefore, LPI technology is a very useful technology for high-throughput shotgun membrane proteomic studies. However, in order to maximize the depth of membrane proteome coverage that can be attained for a given cell type, it is necessary to use multiple sample preparation methods in concert.
Starting Page	539
Ending Page	545
Page Count	7
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	2
Issue Number	5
Journal	Analytical Methods
DOI	10.1039/b9ay00267g
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Mesenchymal stem cell LPI Protease Bone marrow Proteome Shotgun sequencing Protein Nanotechnology Chromatography
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Shotgun proteomics using the itraq isobaric tags. Brief. Funct. Genomics proteomics 5:112–120 (2006).

Comparative proteomic analysis of osteogenic differentiated human adipose tissue and bone marrow-derived stromal cells.

The Extracellular Bone Marrow Microenvironment—A Proteomic Comparison of Constitutive Protein Release by In Vitro Cultured Osteoblasts and Mesenchymal Stem Cells

Expanding the mouse embryonic stem cell proteome: Combining three proteomic approaches

TMT-Based Quantitative Proteomic Analysis Reveals the Effect of Bone Marrow Derived Mesenchymal Stem Cell on Hair Follicle Regeneration

Single-Cell RNA Sequencing of Bone Marrow Mesenchymal Stem Cells from the Elderly People.

Proteomic Profiling of Bone Marrow Mesenchymal Stem Cells Upon TGF-Stimulation

Comparable osteogenic capacity of mesenchymal stem or stromal cells derived from human amnion membrane and bone marrow.

Comparative characteristic study from bone marrow-derived mesenchymal stem cells.

Improving the depth of coverage in membrane proteomic studies through the use of lipid-based protein immobilization technology in parallel with methanol-facilitated solubilisation

Similar Documents

Shotgun proteomics using the itraq isobaric tags. Brief. Funct. Genomics proteomics 5:112–120 (2006).

Comparative proteomic analysis of osteogenic differentiated human adipose tissue and bone marrow-derived stromal cells.

The Extracellular Bone Marrow Microenvironment—A Proteomic Comparison of Constitutive Protein Release by In Vitro Cultured Osteoblasts and Mesenchymal Stem Cells

Expanding the mouse embryonic stem cell proteome: Combining three proteomic approaches

TMT-Based Quantitative Proteomic Analysis Reveals the Effect of Bone Marrow Derived Mesenchymal Stem Cell on Hair Follicle Regeneration

Single-Cell RNA Sequencing of Bone Marrow Mesenchymal Stem Cells from the Elderly People.

Proteomic Profiling of Bone Marrow Mesenchymal Stem Cells Upon TGF-Stimulation

Comparable osteogenic capacity of mesenchymal stem or stromal cells derived from human amnion membrane and bone marrow.

Comparative characteristic study from bone marrow-derived mesenchymal stem cells.

Improving the depth of coverage in membrane proteomic studies through the use of lipid-based protein immobilization technology in parallel with methanol-facilitated solubilisation