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| Content Provider | Royal Society of Chemistry (RSC) |
|---|---|
| Author | Feng, Yi Shen, Lan Zhu, Yunyun Wang, Qiang Cong, Wenjuan Wang, Bin Lin, Xiao |
| Copyright Year | 2013 |
| Abstract | A combinative method using HPLC fingerprinting and quantitative analysis was developed and validated for manufacturer-to-manufacturer quality consistency evaluation of Liuwei Dihuang pills (LDP). Seven bioactive constituents of LDP, including gallic acid, 5-hydroxymethyl furfural, morroniside, paeoniflorin, sweroside, loganin and paeonal, were selected as markers to evaluate the similarities of 20 samples from 16 manufacturers. The similarity values for the 20 LDP samples were all greater than 0.92, indicating that there is a general quality consistency for different manufacturers. Additionally, the 7 selected bioactive compounds were in accordance with the compatibility principle of “the monarch drug, the ministerial drug, the adjunctive drug and the messenger drug” of Traditional Chinese Medicine (TCM) prescription and the multi components quality evaluation requirements of TCM as well. The detection and quantification limits for these 7 bioactive components were all less than 0.11 μg mL−1 and 0.38 μg mL−1, respectively. The mean recovery for all the investigated constituents was acceptable with an accuracy of 98–102%. The intra- and inter-day precisions of this method were less than 4.73%, and stability and repeatability were less than 3.23%. This method is simple and reliable, and also shows that the combination of the chromatographic fingerprint and quantitative analysis offers an efficient solution to quality consistency evaluation of herbal preparations. |
| Starting Page | 2384 |
| Ending Page | 2390 |
| Page Count | 7 |
| File Format | HTM / HTML PDF |
| ISSN | 17599660 |
| Volume Number | 5 |
| Issue Number | 9 |
| Journal | Analytical Methods |
| DOI | 10.1039/c3ay40125a |
| Language | English |
| Publisher | Royal Society of Chemistry |
| Access Restriction | Open |
| Subject Keyword | Liuwei Dihuang Paeoniflorin Acid Fingerprint Furfural Traditional Chinese medicine LDP Loganin High-performance liquid chromatography Chromatography TCM |
| Content Type | Text |
| Resource Type | Article |
| Subject | Analytical Chemistry Engineering Chemical Engineering |
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