NDLI: MicroRNA detection using magnetic separation and zinc-based nanolabels as signal transducers

Please wait, while we are loading the content...

Detection of malachite green and leucomalachite green based on electrochemiluminescence of mono- and bimetallic ruthenium tris-bipyridyl complexes at an Au electrode

Gold nanoparticle–graphene nanohybrid bridged 3-amino-5-mercapto-1,2,4-triazole-functionalized multiwall carbon nanotubes for the simultaneous determination of hydroquinone, catechol, resorcinol and nitrite

A rapid and sensitive spectrofluorometric method for 6-mercaptopurine using CdTe quantum dots

A novel flow injection chemiluminescence method for the determination of ractopamine in urine by using trivalent copper

Gold nanoparticles functionalized poly(3,4-ethylenedioxythiophene) thin film for highly sensitive label free DNA detection

Development and validation of microbial bioassay for the quantification of potency of the antibiotic cefuroxime axetil

Fluorescence resonance energy transfer aptasensor for platelet-derived growth factor detection based on upconversion nanoparticles in 30% blood serum

ZnAl₂O₄-based luminescent marker for gunshot residue identification and ammunition traceability

A combined theoretical and experimental study for the chiral discrimination of naproxen enantiomers by molecular modeling and second-order standard addition method

Determination of aristolochic acids in rat serum by high performance liquid chromatography-Q-TOF tandem mass spectrometry

Core–shell magnetic nanoparticles with surface-imprinted polymer coating as a new adsorbent for solid phase extraction of metronidazole

Determination of alkylamine carbonate nonionic–anion oil displacement agent in oil-field water using HPLC after derivatization with 4-methoxybenzenesulfonyl fluoride

Synthesis of ZnO and its surfactant based electrode for the simultaneous detection of dopamine and ascorbic acid

Centri-voltammetric determination of molybdenum

Assessment of arsenic, chromium, copper and manganese determination in thermal spring waters by electrothermal atomic absorption spectrometry using various chemical modifiers

Stereoselective separation and determination of the triazole fungicide propiconazole in water, soil and grape by normal phase HPLC

Development and validation of a non-aqueous capillary electrophoresis method for simultaneous estimation of mebendazole and levamisole hydrochloride in compound mebendazole tablets

One-pot ultrasonic-electrodeposition of copper–graphene nanoflowers in Ethaline for glucose sensing

An optosensing device for detecting tetracycline antibiotics in honey

Head-space single drop microextraction combined with gas chromatography with an electron capture detector for determination of iodine in infant formulas

Purity assessment of luteolin reference material candidates by coulometric titration method

Enhancement of sensitivity for the spectrophotometric determination of carbaryl using dispersive liquid microextraction combined with dispersive μ-solid phase extraction

Determination of the Raman depolarization ratio in optically active samples

MicroRNA detection using magnetic separation and zinc-based nanolabels as signal transducers

A novel method to measure the radon exhalation rate in only one measurement cycle

Back matter

Back cover

Analytical Methods Vol -4

Analytical Methods Vol -3

Analytical Methods Vol -2

Analytical Methods Vol -1

MicroRNA detection using magnetic separation and zinc-based nanolabels as signal transducers

Content Provider	Royal Society of Chemistry (RSC)
Author	Zhu, Xiaoshan Cowles, Chad L.
Copyright Year	2013
Abstract	In this work, a new microplate based assay for the detection of microRNA (miRNA) is described. The assay uses suspensions of paramagnetic beads immobilized with locked nucleic acid (LNA) oligonucleotide probes to capture miRNA. Poly(A) tails are generated on the captured miRNA using the poly(A) polymerase reaction. Poly(T)30 oligonucleotides hybridize with poly(A) tails and are subsequently labeled with ZnS nanoparticles through biotin–NeutrAvidin chemistry. For signal transduction, zinc ions from the ZnS nanoparticles are released to bind with and activate zinc-ion depleted carbonic anhydrase. The activated kinetics of carbonic anhydrase converts fluorescein diacetate to fluorescein for fluorescence measurement. This work presents a method combining polyadenylation, ion release of nanolabels, and enzyme activation for miRNA detection in a convenient, magnetic bead-based microplate assay format.
Starting Page	801
Ending Page	804
Page Count	4
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	5
Issue Number	3
Journal	Analytical Methods
DOI	10.1039/c2ay25942g
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Microtiter plate Enzyme Paramagnetism Fluorescence Ion Acetate Zinc Polymerase Signal transduction Chemistry MicroRNA Carbonic anhydrase Fluorescein Polyadenylation Oligonucleotide Locked nucleic acid LNA
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A Thermodynamic Study of Zinc Ion Interaction with Bovine Carbonic Anhydrase II at Different Temperatures

Carbonic anhydrase inhibitory properties of phenolic sulfonamides derived from dopamine related compounds

The human carbonic anhydrase isoenzymes I and II inhibitory effects of some hydroperoxides, alcohols, and acetates

Combining Active Carbonic Anhydrase with Nanogels: Enzyme Protection and Zinc Sensing

Chemistry of Peptide-Oligonucleotide Conjugates: A Review

Peroxynitrite inactivates carbonic anhydrase ii by releasing active site zinc ion (2004).

Single-step covalent immobilization of oligonucleotides onto solid surface

Alpha-glucosidase and carbonic anhydrase inhibition studies of Pd(II)-hydrazide complexes

The interaction of acetate and formate with cobalt carbonic anhydrase. An NMR study

MicroRNA detection using magnetic separation and zinc-based nanolabels as signal transducers

Similar Documents

A Thermodynamic Study of Zinc Ion Interaction with Bovine Carbonic Anhydrase II at Different Temperatures

Carbonic anhydrase inhibitory properties of phenolic sulfonamides derived from dopamine related compounds

The human carbonic anhydrase isoenzymes I and II inhibitory effects of some hydroperoxides, alcohols, and acetates

Combining Active Carbonic Anhydrase with Nanogels: Enzyme Protection and Zinc Sensing

Chemistry of Peptide-Oligonucleotide Conjugates: A Review

Peroxynitrite inactivates carbonic anhydrase ii by releasing active site zinc ion (2004).

Single-step covalent immobilization of oligonucleotides onto solid surface

Alpha-glucosidase and carbonic anhydrase inhibition studies of Pd(II)-hydrazide complexes

The interaction of acetate and formate with cobalt carbonic anhydrase. An NMR study

MicroRNA detection using magnetic separation and zinc-based nanolabels as signal transducers