NDLI: A novel urinary oxalate determination method <i>via</i> a catalase model compound with oxalate oxidase

Please wait, while we are loading the content...

Microextraction based on solidification of a floating organic drop followed by electrothermal atomic absorption spectrometry for the determination of ultratraces of lead and cadmium in waters

Determination of atmospheric particulate-phase polycyclic aromatic hydrocarbons from low volume air samples

Observations on comprehensive two dimensional gas chromatography coupled with flame photometric detection for sulfur- and phosphorus-containing compounds

A novel urinary oxalate determination method via a catalase model compound with oxalate oxidase

Rapid and automated sequential determination of ultra-trace long-lived actinides in air filters by inductively coupled plasma mass spectrometry

Quantitative assay of urinary hepcidin using MALDI-TOF mass spectrometry

HPTLC method for estimation of tazarotene in topical gel formulations and in vitro study

Automatic configuration of optimized sample-weighted least-squares support vector machine by particle swarm optimization for multivariate spectral analysis

Weighted partial least squares regression by variable grouping strategy for multivariate calibration of near infrared spectra

Functional analysis of G-protein coupled receptors using a new fluorescein lactone-based intracellular calcium indicator

Back cover

Analytical Methods Vol - 2 Issue - 2

Analytical Methods Vol -1

A novel urinary oxalate determination method via a catalase model compound with oxalate oxidase

Content Provider	Royal Society of Chemistry (RSC)
Author	Zuo, Guanke Jiang, Xiaojun Liu, Hui Zhang, Jingyan
Copyright Year	2010
Abstract	This work describes a novel method of urinary oxalate determination based on a catalase model compound MnL(H2O)2(ClO4)2 (L = bis(2-pyridylmethyl)amino)propionic acid). Urinary oxalate is first decomposed by the oxalate oxidase into carbon dioxide and hydrogen peroxide. The later is then disproportionated into water and oxygen by the catalase model compound forming a color compound, which can be spectrophotometrically monitored. The oxalate concentration of the sample is quantified according to the UV-vis absorbance of the formed color compound. This model compound method maintains the specificity and sensitivity of the conventional enzymatic assay. It has several advantages over the traditional enzymatic method, including low cost, simplicity, large linear range, and adjustable assay time. The model compound method showed a very good linearity in the range of 0.002–20 mmol L−1 oxalate, with a detection limit of 2 μmol L−1 oxalate. The mean urinary oxalate determined by this method was 28.6 μg mL−1, standard deviation (SD) was 1.07 μg mL−1, and variation coefficient (CV) is less than 4%. The results are consistent with that acquired from the enzymatic and HPLC methods. The model compound method also showed that the model compounds of the corresponding enzymes can be an alternative to the enzymes, thus the cost of the methods or assays using the enzymes can be greatly decreased.
Starting Page	254
Ending Page	258
Page Count	5
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	2
Issue Number	3
Journal	Analytical Methods
DOI	10.1039/b9ay00247b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Water Acid Oxygen Hydrogen peroxide Ultraviolet\u2013visible spectroscopy Catalase Carbon dioxide Oxalate oxidase Standard deviation High-performance liquid chromatography
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Electrochemical sensing based on hemin-ordered mesoporous carbon nanocomposites for hydrogen peroxide

Colorimetric determination of total phenolic contents in ionic liquid extracts by paper microzones and digital camera

Preparation, chromatographic evaluation and comparison of cystine- and cysteine-bonded stationary phases

A sensitive HPLC assay for quantitative determination of polyamidoamine dendrimers after pre-column derivatization using FITC

Rapid Determination of Oxalate by an Amperometric Oxalate Oxidase-Based Electrode

Determination of urinary oxalate by high-performance liquid chromatography monitoring with an ultraviolet detector

Fluorescence turn-off detection of hydrogen peroxide and glucose directly using carbon nanodots as probes

Assessment of photo-oxidative alterations to natural organic matter in water using fluorescence excitation emission matrices and liquid chromatography-organic carbon detection techniques

Determination of hydrogen peroxide in workplace air: interferences and method validation

A novel urinary oxalate determination method via a catalase model compound with oxalate oxidase

Similar Documents

Electrochemical sensing based on hemin-ordered mesoporous carbon nanocomposites for hydrogen peroxide

Colorimetric determination of total phenolic contents in ionic liquid extracts by paper microzones and digital camera

Preparation, chromatographic evaluation and comparison of cystine- and cysteine-bonded stationary phases

A sensitive HPLC assay for quantitative determination of polyamidoamine dendrimers after pre-column derivatization using FITC

Rapid Determination of Oxalate by an Amperometric Oxalate Oxidase-Based Electrode

Determination of urinary oxalate by high-performance liquid chromatography monitoring with an ultraviolet detector

Fluorescence turn-off detection of hydrogen peroxide and glucose directly using carbon nanodots as probes

Assessment of photo-oxidative alterations to natural organic matter in water using fluorescence excitation emission matrices and liquid chromatography-organic carbon detection techniques

Determination of hydrogen peroxide in workplace air: interferences and method validation

A novel urinary oxalate determination method via a catalase model compound with oxalate oxidase