NDLI: Genetic Locus Required for Antigenic Maturation of Rhizobium etli CE3 Lipopolysaccharide

Content Provider	PubMed Central
Author	Duelli, Dominik M. Tobin, Andrea Box, Jodie M. Kolli, V. S. Kumar Carlson, Russell W. Noel, K. Dale
Copyright Year	2001
Abstract	Rhizobium etli modifies lipopolysaccharide (LPS) structure in response to environmental signals, such as low pH and anthocyanins. These LPS modifications result in the loss of reactivity with certain monoclonal antibodies. The same antibodies fail to recognize previously isolated R. etli mutant strain CE367, even in the absence of such environmental cues. Chemical analysis of the LPS in strain CE367 demonstrated that it lacked the terminal sugar of the wild-type O antigen, 2,3,4-tri-O-methylfucose. A 3-kb stretch of DNA, designated as lpe3, restored wild-type antigenicity when transferred into CE367. From the sequence of this DNA, five open reading frames were postulated. Site-directed mutagenesis and complementation analysis suggested that the genes were organized in at least two transcriptional units, both of which were required for the production of LPS reactive with the diagnostic antibodies. Growth in anthocyanins or at low pH did not alter the specific expression of gusA from the transposon insertion of mutant CE367, nor did the presence of multiple copies of lpe3 situated behind a strong, constitutive promoter prevent epitope changes induced by these environmental cues. Mutations of the lpe genes did not prevent normal nodule development on Phaseolus vulgaris and had very little effect on the occupation of nodules in competition with the wild-type strain.
Related Links	http://dx.doi.org/10.1128/jb.183.20.6054-6064.2001
Ending Page	6064
Page Count	11
Starting Page	6054
File Format	PDF
ISSN	00219193
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	20
Volume Number	183
Language	English
Publisher	American Society for Microbiology
Publisher Date	2001-10-15
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Molecular Biology Microbiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

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Genetic Basis for Rhizobium etli CE3 O-Antigen O-Methylated Residues That Vary According to Growth Conditions▿

Elucidation of the 3-O-Deacylase Gene, pagL, Required for the Removal of Primary β-Hydroxy Fatty Acid from the Lipid A in the Nitrogen-fixing Endosymbiont Rhizobium etli CE3

Roles of Predicted Glycosyltransferases in the Biosynthesis of the Rhizobium etli CE3 O Antigen

Identification of a Plasmid-Borne Locus in Rhizobium etli KIM5s Involved in Lipopolysaccharide O-Chain Biosynthesis and Nodulation of Phaseolus vulgaris

The extracellular proteome of Rhizobium etli CE3 in exponential and stationary growth phase

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Elucidation of the 3-O-Deacylase Gene, pagL, Required for the Removal of Primary β-Hydroxy Fatty Acid from the Lipid A in the Nitrogen-fixing Endosymbiont Rhizobium etli CE3*

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