NDLI: Genetic Requirements for Induction of Germination of Spores of Bacillus subtilis by Ca2+-Dipicolinate

Content Provider	PubMed Central
Author	Madan, Paidhungat Katerina, Ragkousi Peter, Setlow
Copyright Year	2001
Abstract	Dormant Bacillus subtilis spores can be induced to germinate by nutrients, as well as by nonmetabolizable chemicals, such as a 1:1 chelate of Ca2+ and dipicolinic acid (DPA). Nutrients bind receptors in the spore, and this binding triggers events in the spore core, including DPA excretion and rehydration, and also activates hydrolysis of the surrounding cortex through mechanisms that are largely unknown. As Ca2+-DPA does not require receptors to induce spore germination, we asked if this process utilizes other proteins, such as the putative cortex-lytic enzymes SleB and CwlJ, that are involved in nutrient-induced germination. We found that Ca2+-DPA triggers germination by first activating CwlJ-dependent cortex hydrolysis; this mechanism is different from nutrient-induced germination where cortex hydrolysis is not required for the early germination events in the spore core. Nevertheless, since nutrients can induce release of the spore's DPA before cortex hydrolysis, we examined if the DPA excreted from the core acts as a signal to activate CwlJ in the cortex. Indeed, endogenous DPA is required for nutrient-induced CwlJ activation and this requirement was partially remedied by exogenous Ca2+-DPA. Our findings thus define a mechanism for Ca2+-DPA-induced germination and also provide the first definitive evidence for a signaling pathway that activates cortex hydrolysis in response to nutrients.
Related Links	http://dx.doi.org/10.1128/jb.183.16.4886-4893.2001
Ending Page	4893
Page Count	8
Starting Page	4886
File Format	PDF
ISSN	00219193
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	16
Volume Number	183
Language	English
Publisher	American Society for Microbiology
Publisher Date	2001-08-15
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Molecular Biology Microbiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Identification of a New Gene Essential for Germination of Bacillus subtilis Spores with Ca2+-Dipicolinate

Germination of Spores of Bacillus stearothermophilus Induced by Analogues of Dipicolinate Di-Anion

Germination of Spores of Bacillus megaterium with Divalent Metal-Dipicolinate Chelates

Germination Requirements of Bacillus macerans Spores

Role of GerD in Germination of Bacillus subtilis Spores▿

Macromolecular Syntheses During Germination and Outgrowth of Bacillus subtilis Spores

Ultraviolet Sensitivity of Bacillus subtilis Spores upon Germination and Outgrowth

Ultrastructural Analysis During Germination and Outgrowth of Bacillus subtilis Spores

HtrC Is Involved in Proteolysis of YpeB during Germination of Bacillus anthracis and Bacillus subtilis Spores

Genetic Requirements for Induction of Germination of Spores of Bacillus subtilis by Ca2+-Dipicolinate

Similar Documents

Identification of a New Gene Essential for Germination of Bacillus subtilis Spores with Ca2+-Dipicolinate

Germination of Spores of Bacillus stearothermophilus Induced by Analogues of Dipicolinate Di-Anion

Germination of Spores of Bacillus megaterium with Divalent Metal-Dipicolinate Chelates

Germination Requirements of Bacillus macerans Spores

Role of GerD in Germination of Bacillus subtilis Spores▿

Macromolecular Syntheses During Germination and Outgrowth of Bacillus subtilis Spores

Ultraviolet Sensitivity of Bacillus subtilis Spores upon Germination and Outgrowth

Ultrastructural Analysis During Germination and Outgrowth of Bacillus subtilis Spores

HtrC Is Involved in Proteolysis of YpeB during Germination of Bacillus anthracis and Bacillus subtilis Spores

Genetic Requirements for Induction of Germination of Spores of Bacillus subtilis by Ca2+-Dipicolinate