NDLI: Analysis of Urease Expression in Actinomyces naeslundii WVU45

Content Provider	PubMed Central
Author	Morou-bermudez, Evangelia Burne, Robert A.
Editor	Clements, J. D.
Copyright Year	2000
Abstract	The hydrolysis of urea by ureases of oral bacteria in dental plaque can cause a considerable increase in plaque pH, which can inhibit the development of dental caries. There is also indirect evidence that urea metabolism may promote the formation of calculus and that ammonia release from urea could exacerbate periodontal diseases. Actinomyces naeslundii, an early colonizer of the oral cavity and a numerically significant plaque constituent, demonstrates comparatively low levels of urease activity on isolation, so this organism has not been considered a major contributor to total oral urease activity. In this study it was observed that urease activity and urease-specific mRNA levels in A. naeslundii WVU45 can increase up to 50-fold during growth under nitrogen-limiting conditions. Using primer extension analysis, a putative, proximal, nitrogen-regulated promoter of the A. naeslundii urease gene cluster was identified. The functionality and nitrogen responsiveness of this promoter were confirmed using reporter gene fusions and 5′ deletion analysis. The data indicated that regulation of urease expression by nitrogen availability in A. naeslundii may require a positive transcriptional activator. Plaque bacteria may experience nitrogen limitation when carbohydrates are present in excess. Therefore, based on the results of this study and in contrast to previous beliefs, strains of A. naeslundii may have the potential to be significant contributors to total plaque ureolysis, particularly during periods when there is an increased risk for caries development.
Starting Page	6670
File Format	PDF
ISSN	10985522
e-ISSN	10985522
Journal	Infection and Immunity
Issue Number	12
Volume Number	68
Language	English
Publisher	American Society for Microbiology
Publisher Date	2000-12-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Parasitology Immunology Microbiology

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Structural preferences of beta-galactoside-reactive lectins on Actinomyces viscosus T14V and Actinomyces naeslundii WVU45.

Exclusive presence of lactose-sensitive fimbriae on a typical strain (WVU45) of Actinomyces naeslundii.

Putative glycoprotein and glycolipid polymorphonuclear leukocyte receptors for the Actinomyces naeslundii WVU45 fimbrial lectin.

Genetic and Physiologic Characterization of Urease of Actinomyces naeslundii

Mannose-contaminating agglutinin for Actinomyces viscosus and Actinomyces naeslundii.

Binding of Actinomyces naeslundii to glycosphingolipids.

Sialidase-Enhanced Lectin-Like Mechanism for Actinomyces viscosus and Actinomyces naeslundii Hemagglutination

Roles of Fructosyltransferase and Levanase-Sucrase of Actinomyces naeslundii in Fructan and Sucrose Metabolism

Characterization of the Fructosyltransferase Gene of Actinomyces naeslundii WVU45

Analysis of Urease Expression in Actinomyces naeslundii WVU45

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Structural preferences of beta-galactoside-reactive lectins on Actinomyces viscosus T14V and Actinomyces naeslundii WVU45.

Exclusive presence of lactose-sensitive fimbriae on a typical strain (WVU45) of Actinomyces naeslundii.

Putative glycoprotein and glycolipid polymorphonuclear leukocyte receptors for the Actinomyces naeslundii WVU45 fimbrial lectin.

Genetic and Physiologic Characterization of Urease of Actinomyces naeslundii

Mannose-contaminating agglutinin for Actinomyces viscosus and Actinomyces naeslundii.

Binding of Actinomyces naeslundii to glycosphingolipids.

Sialidase-Enhanced Lectin-Like Mechanism for Actinomyces viscosus and Actinomyces naeslundii Hemagglutination

Roles of Fructosyltransferase and Levanase-Sucrase of Actinomyces naeslundii in Fructan and Sucrose Metabolism

Characterization of the Fructosyltransferase Gene of Actinomyces naeslundii WVU45

Analysis of Urease Expression in Actinomyces naeslundii WVU45