NDLI: Probing the Function of Bordetella bronchiseptica Adenylate Cyclase Toxin by Manipulating Host Immunity

Content Provider	PubMed Central
Author	Harvill, Eric T. Cotter, Peggy A. Yuk, Ming Huam Miller, Jeff F.
Editor	Orndorff, P. E.
Copyright Year	1999
Abstract	We have examined the role of adenylate cyclase-hemolysin (CyaA) by constructing an in-frame deletion in the Bordetella bronchiseptica cyaA structural gene and comparing wild-type and cyaA deletion strains in natural host infection models. Both the wild-type strain RB50 and its adenylate cyclase toxin deletion (ΔcyaA) derivative efficiently establish persistent infections in rabbits, rats, and mice following low-dose inoculation. In contrast, an inoculation protocol that seeds the lower respiratory tract revealed significant differences in bacterial numbers and in polymorphonuclear neutrophil recruitment in the lungs from days 5 to 12 postinoculation. We next explored the effects of disarming specific aspects of the immune system on the relative phenotypes of wild-type and ΔcyaA bacteria. SCID, SCID-beige, or RAG-1−/− mice succumbed to lethal systemic infection following high- or low-dose intranasal inoculation with the wild-type strain but not the ΔcyaA mutant. Mice rendered neutropenic by treatment with cyclophosphamide or by knockout mutation in the granulocyte colony-stimulating factor locus were highly susceptible to lethal infection by either wild-type or ΔcyaA strains. These results reveal the significant role played by neutrophils early in B. bronchiseptica infection and by acquired immunity at later time points and suggest that phagocytic cells are a primary in vivo target of the Bordetella adenylate cyclase toxin.
Starting Page	1493
File Format	PDF
ISSN	10985522
e-ISSN	10985522
Journal	Infection and Immunity
Issue Number	3
Volume Number	67
Language	English
Publisher	American Society for Microbiology
Publisher Date	1999-03-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Parasitology Immunology Microbiology

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Bordetella pertussis adenylate cyclase toxin: intoxication of host cells by bacterial invasion.

Virulence of Bordetella bronchiseptica: role of adenylate cyclase-hemolysin.

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Stimulation of Bordetella pertussis Adenylate Cyclase Toxin Intoxication by Its Hemolysin Domain

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Probing the Function of Bordetella bronchiseptica Adenylate Cyclase Toxin by Manipulating Host Immunity

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