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| Content Provider | PubMed Central |
|---|---|
| Author | Breukels, Mijke A. Rijkers, Ger T. Voorhorst-ogink, Marleen M. Zegers, Ben J. M. |
| Editor | Moore, R. N. |
| Copyright Year | 1999 |
| Abstract | An in vitro culture system for the induction of an antipolysaccharide response was used to study the cellular interactions which determine the magnitude and nature of this B-lymphocyte response. Healthy adult volunteers were vaccinated with the Haemophilus influenzae type b polysaccharide (PRP)-tetanus toxoid (TT) conjugate vaccine. Optimal in vitro anti-PRP and anti-TT antibody responses were obtained when B cells were cultured with equal amounts of T cells. The in vitro response is antigen dependent and antigen specific. Culturing with PRP mixed with TT in the presence of T cells induces the highest number of anti-PRP antibody-secreting cells (ASC) (128.4 ×/÷ 15.9 [geometric mean ×/÷ standard deviation] immunoglobulin M [IgM] anti-PRP ASC/106 cells; 9.3 ×/÷ 7.6 IgG anti-PRP ASC/106 cells). Culturing without T cells induced no anti-PRP ASC; culturing with only PRP, in the presence of T cells, yielded low numbers of anti-PRP ASC (3.7 ×/÷ 5.2 IgM anti-PRP ASC/106 cells and 1.2 ×/÷ 2.2 IgG anti-PRP ASC/106 cells). Transwell studies showed that the requirements for the antibody response against the polysaccharide are different from those of an antiprotein response. Cytokines formed as a consequence of contact between protein-specific B and T cells were on their own not sufficient to activate TT-specific B cells (8.4 ×/÷ 1.4 anti-TT ASC/106 cells); direct contact between T and B cells appeared to be an absolute requirement. However, physical contact between B and T cells in one compartment of the Transwell system resulted in the release of soluble factors able to stimulate B cells in the other compartment to secrete antipolysaccharide antibodies (164 ×/÷ 1.6 anti-PRP ASC/106 cells). |
| Starting Page | 789 |
| File Format | |
| ISSN | 10985522 |
| e-ISSN | 10985522 |
| Journal | Infection and Immunity |
| Issue Number | 2 |
| Volume Number | 67 |
| Language | English |
| Publisher | American Society for Microbiology |
| Publisher Date | 1999-02-01 |
| Access Restriction | Open |
| Rights Holder | American Society for Microbiology |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Infectious Diseases Parasitology Immunology Microbiology |
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