NDLI: Bacillus subtilis NhaC, an Na+/H+ Antiporter, Influences Expression of the phoPR Operon and Production of Alkaline Phosphatases

Content Provider	PubMed Central
Author	Prágai, Zoltán Caroline, Eschevins Bron, Sierd Harwood, Colin R.
Copyright Year	2001
Abstract	When Bacillus subtilis is subjected to phosphate starvation, genes of the Pho regulon are either induced or repressed. Among those induced are genes encoding alkaline phosphatases (APases). A set of isogenic mutants, with a β-galactosidase gene transcriptionally fused to the inactivated target gene, was used to identify genes that influence the operation of the Pho regulon. One such gene was nhaC (previously yheL). In the absence of NhaC, growth and APase production were enhanced, while the production of other non-Pho-regulon secretory proteins (proteases and α-amylase) did not change. The influence of NhaC on growth, APase synthesis, and its own expression was dependent on the external Na+ concentration. Other monovalent cations such as Li+ or K+ had no effect. We propose a role for NhaC in the uptake of Na+. nhaC appears to be encoded by a monocistronic operon and, contrary to previous reports, is not in the same transcriptional unit as yheK, the gene immediately upstream. The increase in APase production was dependent on an active PhoR, the sensor kinase of the two-component system primarily responsible for controlling the Pho regulon. Transcriptional fusions showed that the phoPR operon and both phoA (encoding APaseA) and phoB (encoding APaseB) were hyperinduced in the absence of NhaC and repressed when this protein was overproduced. This suggests that NhaC effects APase production via phoPR.
Related Links	http://dx.doi.org/10.1128/jb.183.8.2505-2515.2001
Ending Page	2515
Page Count	11
Starting Page	2505
File Format	PDF
ISSN	00219193
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	8
Volume Number	183
Language	English
Publisher	American Society for Microbiology
Publisher Date	2001-04-15
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Molecular Biology Microbiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Role of the nhaC-encoded Na+/H+ antiporter of alkaliphilic Bacillus firmus OF4.

Transcriptional Regulation of the phoPR Operon in Bacillus subtilis

Complex Formation by the mrpABCDEFG Gene Products, Which Constitute a Principal Na+/H+ Antiporter in Bacillus subtilis ▿

Function of a Principal Na+/H+ Antiporter, ShaA, Is Required for Initiation of Sporulation in Bacillus subtilis

Autoinduction of Bacillus subtilis phoPR Operon Transcription Results from Enhanced Transcription from EσA- and EσE-Responsive Promoters by Phosphorylated PhoP

GerN, an Endospore Germination Protein of Bacillus cereus, Is an Na+/H+-K+ Antiporter

Purification and properties of two membrane alkaline phosphatases from Bacillus subtilis 168.

Structure and expression of the Bacillus subtilis sin operon.

Role and expression of the Bacillus subtilis rodC operon.

Bacillus subtilis NhaC, an Na+/H+ Antiporter, Influences Expression of the phoPR Operon and Production of Alkaline Phosphatases

Similar Documents

Role of the nhaC-encoded Na+/H+ antiporter of alkaliphilic Bacillus firmus OF4.

Transcriptional Regulation of the phoPR Operon in Bacillus subtilis

Complex Formation by the mrpABCDEFG Gene Products, Which Constitute a Principal Na+/H+ Antiporter in Bacillus subtilis ▿

Function of a Principal Na+/H+ Antiporter, ShaA, Is Required for Initiation of Sporulation in Bacillus subtilis

Autoinduction of Bacillus subtilis phoPR Operon Transcription Results from Enhanced Transcription from EσA- and EσE-Responsive Promoters by Phosphorylated PhoP

GerN, an Endospore Germination Protein of Bacillus cereus, Is an Na+/H+-K+ Antiporter

Purification and properties of two membrane alkaline phosphatases from Bacillus subtilis 168.

Structure and expression of the Bacillus subtilis sin operon.

Role and expression of the Bacillus subtilis rodC operon.

Bacillus subtilis NhaC, an Na+/H+ Antiporter, Influences Expression of the phoPR Operon and Production of Alkaline Phosphatases