NDLI: Mobile Cytochrome c 2 and Membrane-Anchored Cytochrome c y Are Both Efficient Electron Donors to the cbb 3- and aa 3-Type Cytochrome c Oxidases during Respiratory Growth of Rhodobacter sphaeroides

Content Provider	PubMed Central
Author	Daldal, Fevzi Mandaci, Sevnur Winterstein, Christine Myllykallio, Hannu Duyck, Kristen Zannoni, Davide
Copyright Year	2001
Abstract	We have recently established that the facultative phototrophic bacterium Rhodobacter sphaeroides, like the closely related Rhodobacter capsulatus species, contains both the previously characterized mobile electron carrier cytochrome c 2 (cyt c 2) and the more recently discovered membrane-anchored cyt c y. However, R. sphaeroides cyt c y, unlike that of R. capsulatus, is unable to function as an efficient electron carrier between the photochemical reaction center and the cyt bc 1 complex during photosynthetic growth. Nonetheless, R. sphaeroides cyt c y can act at least in R. capsulatus as an electron carrier between the cyt bc 1 complex and the cbb 3-type cyt c oxidase (cbb 3-Cox) to support respiratory growth. Since R. sphaeroides harbors both a cbb 3-Cox and an aa 3-type cyt c oxidase (aa 3-Cox), we examined whether R. sphaeroides cyt c y can act as an electron carrier to either or both of these respiratory terminal oxidases. R. sphaeroides mutants which lacked either cyt c 2 or cyt c y and either the aa 3-Cox or the cbb 3-Cox were obtained. These double mutants contained linear respiratory electron transport pathways between the cyt bc 1 complex and the cyt c oxidases. They were characterized with respect to growth phenotypes, contents of a-, b-, and c-type cytochromes, cyt c oxidase activities, and kinetics of electron transfer mediated by cyt c 2 or cyt c y. The findings demonstrated that both cyt c 2 and cyt c y are able to carry electrons efficiently from the cyt bc 1 complex to either the cbb 3-Cox or the aa 3-Cox. Thus, no dedicated electron carrier for either of the cyt c oxidases is present in R. sphaeroides. However, under semiaerobic growth conditions, a larger portion of the electron flow out of the cyt bc 1 complex appears to be mediated via the cyt c 2-to-cbb 3-Cox and cyt cy -to-cbb 3-Cox subbranches. The presence of multiple electron carriers and cyt c oxidases with different properties that can operate concurrently reveals that the respiratory electron transport pathways of R. sphaeroides are more complex than those of R. capsulatus.
Related Links	http://dx.doi.org/10.1128/jb.183.6.2013-2024.2001
Ending Page	2024
Page Count	12
Starting Page	2013
File Format	PDF
ISSN	00219193
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	6
Volume Number	183
Language	English
Publisher	American Society for Microbiology
Publisher Date	2001-03-15
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Molecular Biology Microbiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

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