NDLI: The Last RNA-Binding Repeat of the Escherichia coli Ribosomal Protein S1 Is Specifically Involved in Autogenous Control

Content Provider	PubMed Central
Author	Boni, Irina V. Artamonova, Valentina S. Dreyfus, Marc
Copyright Year	2000
Abstract	The ssyF29 mutation, originally selected as an extragenic suppressor of a protein export defect, has been mapped within the rpsA gene encoding ribosomal protein S1. Here, we examine the nature of this mutation and its effect on translation. Sequencing of the rpsA gene from the ssyF mutant has revealed that, due to an IS10R insertion, its product lacks the last 92 residues of the wild-type S1 protein corresponding to one of the four homologous repeats of the RNA-binding domain. To investigate how this truncation affects translation, we have created two series of Escherichia coli strains (rpsA + and ssyF) bearing various translation initiation regions (TIRs) fused to the chromosomal lacZ gene. Using a β-galactosidase assay, we show that none of these TIRs differ in activity between ssyF and rpsA + cells, except for the rpsA TIR: the latter is stimulated threefold in ssyF cells, provided it retains at least ca. 90 nucleotides upstream of the start codon. Similarly, the activity of this TIR can be severely repressed in trans by excess S1, again provided it retains the same minimal upstream sequence. Thus, the ssyF stimulation requires the presence of the rpsA translational autogenous operator. As an interpretation, we propose that the ssyF mutation relieves the residual repression caused by normal supply of S1 (i.e., that it impairs autogenous control). Thus, the C-terminal repeat of the S1 RNA-binding domain appears to be required for autoregulation, but not for overall mRNA recognition.
Starting Page	5872
File Format	PDF
ISSN	10985530
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	20
Volume Number	182
Language	English
Publisher	American Society for Microbiology
Publisher Date	2000-10-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Specific Binding of Escherichia coli Ribosomal Protein S1 to boxA Transcriptional Antiterminator RNA

Autogenous control is not sufficient to ensure steady-state growth rate-dependent regulation of the S10 ribosomal protein operon of Escherichia coli.

Autogenous control of PspF, a constitutively active enhancer-binding protein of Escherichia coli.

The binding site for ribosomal protein L2 within 23S ribosomal RNA of Escherichia coli.

Levels of ribosomal protein S1 and elongation factor G in the growth cycle of Escherichia coli.

Binding of ribosomal protein S1 of Escherichia coli to the 3' end of 16S rRNA.

Interaction of Escherichia coli ribosomal protein S1 with ribosomes.

Escherichia coli ribosomal protein S1 enhances the kinetics of ribosome biogenesis and RNA decay

Escherichia coli ribosomal protein S1 has two polynucleotide binding sites.

The Last RNA-Binding Repeat of the Escherichia coli Ribosomal Protein S1 Is Specifically Involved in Autogenous Control

Similar Documents

Specific Binding of Escherichia coli Ribosomal Protein S1 to boxA Transcriptional Antiterminator RNA

Autogenous control is not sufficient to ensure steady-state growth rate-dependent regulation of the S10 ribosomal protein operon of Escherichia coli.

Autogenous control of PspF, a constitutively active enhancer-binding protein of Escherichia coli.

The binding site for ribosomal protein L2 within 23S ribosomal RNA of Escherichia coli.

Levels of ribosomal protein S1 and elongation factor G in the growth cycle of Escherichia coli.

Binding of ribosomal protein S1 of Escherichia coli to the 3' end of 16S rRNA.

Interaction of Escherichia coli ribosomal protein S1 with ribosomes.

Escherichia coli ribosomal protein S1 enhances the kinetics of ribosome biogenesis and RNA decay

Escherichia coli ribosomal protein S1 has two polynucleotide binding sites.

The Last RNA-Binding Repeat of the Escherichia coli Ribosomal Protein S1 Is Specifically Involved in Autogenous Control