NDLI: Replacement of Vegetative ςA by Sporulation-Specific ςF as a Component of the RNA Polymerase Holoenzyme in Sporulating Bacillus subtilis

Content Provider	PubMed Central
Author	Lord, Matthew Daniela, Barillà Yudkin, Michael D.
Copyright Year	1999
Abstract	Soon after asymmetric septation in sporulating Bacillus subtilis cells, ςF is liberated in the prespore from inhibition by SpoIIAB. To initiate transcription from its cognate promoters, ςF must compete with ςA, the housekeeping sigma factor in the predivisional cell, for binding to core RNA polymerase (E). To estimate the relative affinity of E for ςA and ςF, we made separate mixtures of E with each of the two sigma factors, allowed reconstitution of the holoenzyme, and measured the concentration of free E remaining in each mixture. The affinity of E for ςF was found to be about 25-fold lower than that for ςA. We used quantitative Western blotting to estimate the concentrations of E, ςA, and ςF in sporulating cells. The cellular concentrations of E and ςA were both about 7.5 μM, and neither changed significantly during the first 3 h of sporulation. The concentration of ςF was extremely low at the beginning of sporulation, but it rose rapidly to a peak after about 2 h. At its peak, the concentration of ςF was some twofold higher than that of ςA. This difference in concentration cannot adequately account for the replacement of ςA holoenzyme by ςF holoenzyme in the prespore, and it seems that some further mechanism—perhaps the synthesis or activation of an anti-ςA factor—must be responsible for this replacement.
Starting Page	2346
File Format	PDF
ISSN	10985530
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	8
Volume Number	181
Language	English
Publisher	American Society for Microbiology
Publisher Date	1999-04-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

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