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| Content Provider | PubMed Central |
|---|---|
| Author | Milton, Donald K. Alwis, K. Udeni Fisette, Leslie Michael, Muilenberg |
| Copyright Year | 2001 |
| Abstract | (1→3)-β-d-Glucans have been recognized as a potential causative agent responsible for bioaerosol-induced respiratory symptoms observed in both indoor and occupational environments. A specific enzyme immunoassay was developed to quantify (1→6) branched, (1→3)-β-d-glucans in environmental samples. The assay was based on the use of a high-affinity receptor (galactosyl ceramide) specific for (1→3)-β-d-glucans as a capture reagent and a monoclonal antibody specific for fungal cell wall β-d-glucans as a detector reagent. The assay was highly specific for (1→6) branched, (1→3)-β-d-glucans (such as that from Saccharomyces cerevisiae) and did not show any response at 200 ng/ml to curdlan, laminarin, pustulan, dextran, mannan, carboxymethyl cellulose, and endotoxins. The detection level was 0.8 ng/ml for baker's yeast glucan and Betafectin. A coefficient of variation of 7.8% was obtained for (1→3)-β-d-glucans in house dust samples. Metal working fluids spiked with (1→3)-β-d-glucans inhibited the glucan assay. Because the assay is specific for (1→6) branched, (1→3)-β-d-glucans and is sensitive and reproducible, it will be useful for the investigation of health effects from exposure to this class of biologically active molecules. |
| Related Links | http://dx.doi.org/10.1128/aem.67.12.5420-5424.2001 |
| Ending Page | 5424 |
| Page Count | 5 |
| Starting Page | 5420 |
| File Format | |
| ISSN | 00992240 |
| e-ISSN | 10985336 |
| Journal | Applied and Environmental Microbiology |
| Issue Number | 12 |
| Volume Number | 67 |
| Language | English |
| Publisher | American Society for Microbiology |
| Publisher Date | 2001-12-01 |
| Access Restriction | Open |
| Rights Holder | American Society for Microbiology |
| Subject Keyword | Biotechnology Food Science Ecology Applied Microbiology and Biotechnology Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Ecology Food Science Applied Microbiology and Biotechnology Biotechnology |
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