NDLI: Slaughter Pigs Are Commonly Infected by Closely Related but Distinct Gastric Ulcerative Lesion-Inducing Gastrospirilla

Content Provider	PubMed Central
Author	Roosendaal, Robert Vos, Jan H. Thijs, Roumen Vugt, René Van Giovanni, Cattoli Bart, Aldert Klaasen, Henricus L. B. M. Kuipers, Ernst J. Vandenbroucke-grauls, Christina M. J. E. Kusters, Johannes G.
Copyright Year	2000
Abstract	An association between (unculturable) gastrospirillum-like organisms (GLO) and ulcerative lesions in the pars oesophagea in stomachs of swine has been claimed. In dogs GLO detected by microscopy may represent several Helicobacter species or subspecies. Therefore we investigated which Helicobacter spp. are present in stomachs of swine and their possible association with ulcerative lesions of the pars oesophagea. The presence of Helicobacter spp. in the antrum and pars oesophagea in 122 stomachs of slaughter swine was determined by microscopy (n = 122), by culture on selective and nonselective media (n = 112), and by a genus-specific 16S ribosomal DNA (rDNA) PCR (n = 80). GLO could not be cultured. Phylogenetic analysis of 43 16S rDNA fragments (out of 54 PCR-positive biopsy specimens) revealed the presence of Helicobacter heilmannii type 1 in 42 of them. This correlated with the presence of bacteria with GLO morphology. Helicobacter bilis 16S rDNA was amplified directly from one sample harboring bacteria with H. bilis morphology. The association between Helicobacter spp. and gastric lesions was investigated with a second group of 41 pigs with (n = 21 cases) or without (n = 20 controls) gastric lesions. Fifteen of the 21 cases were positive by PCR or microscopy, compared to 7 of 20 of the controls (P = 0.03). 16S rDNA sequence analysis of 7 of 14 PCR-positive cases revealed the presence of H. heilmannii type 1. Microscopy showed bacteria with GLO morphology. One sample (cases) was culture negative but PCR positive for Helicobacter pullorum-related 16S rDNA. In conclusion, our findings indicate that H. heilmannii type 1 is the predominant Helicobacter spp. in the stomachs of pigs and that its presence is associated with ulcerative lesions in the pars oesophagea.
Starting Page	2661
File Format	PDF
ISSN	1098660X
e-ISSN	1098660X
Journal	Journal of Clinical Microbiology
Issue Number	7
Volume Number	38
Language	English
Publisher	American Society for Microbiology
Publisher Date	2000-07-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Microbiology (medical)

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Two Closely Related but Distinct Retroviruses Are Associated with Walleye Discrete Epidermal Hyperplasia

Captive Rhesus Monkeys (Macaca mulatta) Are Commonly Infected with Helicobacter cinaedi

African Great Apes Are Naturally Infected with Roseoloviruses Closely Related to Human Herpesvirus 7

Human cord blood mononuclear cells are preferentially infected by non-syncytium-inducing, macrophage-tropic human immunodeficiency virus type 1 isolates.

Lone Star Tick-Infecting Borreliae Are Most Closely Related to the Agent of Bovine Borreliosis

African Great Apes Are Naturally Infected with Polyomaviruses Closely Related to Merkel Cell Polyomavirus▿ †

Rapid species identification of "Streptococcus milleri" strains by line blot hybridization: identification of a distinct 16S rRNA population closely related to Streptococcus constellatus.

Fusarium circinatum isolates from northern Spain are commonly infected by three distinct mitoviruses

Blastocystis Isolates from a Pig and a Horse Are Closely Related to Blastocystis hominis

Slaughter Pigs Are Commonly Infected by Closely Related but Distinct Gastric Ulcerative Lesion-Inducing Gastrospirilla

Similar Documents

Two Closely Related but Distinct Retroviruses Are Associated with Walleye Discrete Epidermal Hyperplasia

Captive Rhesus Monkeys (Macaca mulatta) Are Commonly Infected with Helicobacter cinaedi

African Great Apes Are Naturally Infected with Roseoloviruses Closely Related to Human Herpesvirus 7

Human cord blood mononuclear cells are preferentially infected by non-syncytium-inducing, macrophage-tropic human immunodeficiency virus type 1 isolates.

Lone Star Tick-Infecting Borreliae Are Most Closely Related to the Agent of Bovine Borreliosis

African Great Apes Are Naturally Infected with Polyomaviruses Closely Related to Merkel Cell Polyomavirus▿ †

Rapid species identification of "Streptococcus milleri" strains by line blot hybridization: identification of a distinct 16S rRNA population closely related to Streptococcus constellatus.

Fusarium circinatum isolates from northern Spain are commonly infected by three distinct mitoviruses

Blastocystis Isolates from a Pig and a Horse Are Closely Related to Blastocystis hominis

Slaughter Pigs Are Commonly Infected by Closely Related but Distinct Gastric Ulcerative Lesion-Inducing Gastrospirilla